Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4546964..4547774 Replicon chromosome
Accession NZ_CP086285
Organism Klebsiella pneumoniae strain Kp1

Toxin (Protein)


Gene name KacT Uniprot ID A0A6G4MHE5
Locus tag LL004_RS22415 Protein ID WP_065801222.1
Coordinates 4546964..4547497 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LL004_RS22420 Protein ID WP_002887278.1
Coordinates 4547508..4547774 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL004_RS22410 4545795..4546916 + 1122 WP_004214138.1 cupin domain-containing protein -
LL004_RS22415 4546964..4547497 - 534 WP_065801222.1 type II toxin-antitoxin system toxin KacT Toxin
LL004_RS22420 4547508..4547774 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LL004_RS22425 4547877..4549310 - 1434 WP_065801221.1 Cu(+)/Ag(+) sensor histidine kinase -
LL004_RS22430 4549300..4549983 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
LL004_RS22435 4550155..4551540 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
LL004_RS22440 4551558..4551902 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19868.73 Da        Isoelectric Point: 5.2614

>T222897 WP_065801222.1 NZ_CP086285:c4547497-4546964 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRSYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKVFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T222897 NZ_CP118152:698598-698888 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT222897 WP_002887278.1 NZ_CP086285:c4547774-4547508 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT222897 NZ_CP118152:698318-698596 [Pseudomonas chlororaphis]
CTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCATCGCTCGGCGTATCGA
GCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCGTGAACACAGGCGCTG
GCTACCGCCTCTATTTCACGGTTCGCGGCAATCCCCTCATCGTCCTGCTGCTGGGTGGCGACAAGTCCAGCCAGCCTGCC
GATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4MHE5

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References