Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3310468..3311101 Replicon chromosome
Accession NZ_CP086265
Organism Ralstonia sp. RS647

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag LGV81_RS15725 Protein ID WP_016725172.1
Coordinates 3310468..3310881 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag LGV81_RS15730 Protein ID WP_011002949.1
Coordinates 3310868..3311101 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV81_RS15705 (LGV81_15680) 3305846..3307582 + 1737 WP_019718916.1 ABC transporter substrate-binding protein -
LGV81_RS15710 (LGV81_15685) 3307744..3309240 + 1497 WP_016725175.1 glycerol kinase GlpK -
LGV81_RS15715 (LGV81_15690) 3309305..3309676 - 372 WP_016725174.1 hypothetical protein -
LGV81_RS15720 (LGV81_15695) 3309867..3310493 + 627 WP_016725173.1 DUF1415 domain-containing protein -
LGV81_RS15725 (LGV81_15700) 3310468..3310881 - 414 WP_016725172.1 PIN domain-containing protein Toxin
LGV81_RS15730 (LGV81_15705) 3310868..3311101 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LGV81_RS15735 (LGV81_15710) 3311158..3312765 - 1608 WP_020832850.1 glucan biosynthesis protein -
LGV81_RS15740 (LGV81_15715) 3312960..3314210 + 1251 WP_265069770.1 AGE family epimerase/isomerase -
LGV81_RS15745 (LGV81_15720) 3314218..3315513 + 1296 WP_265069771.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T222883 WP_016725172.1 NZ_CP086265:c3310881-3310468 [Ralstonia sp. RS647]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T222883 NZ_CP118150:c1805498-1805316 [Pseudomonas chlororaphis]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCACCCTTT
CAGGCCCCCCAGTAAACCGGGGCTGGTGACAGTTCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT222883 WP_011002949.1 NZ_CP086265:c3311101-3310868 [Ralstonia sp. RS647]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT222883 NZ_CP118150:c1805283-1804876 [Pseudomonas chlororaphis]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGTGACGACCTCGATGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCGCCATCCCGTCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCTCAGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCGAAGAGCCGCTCGGGTTTTCTCGCTTCGGCTGCATTGAAGGTATTGCAGC
AAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References