Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 5142..5635 Replicon plasmid pB-9427-3
Accession NZ_CP086262
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag LMH79_RS26635 Protein ID WP_001230709.1
Coordinates 5142..5396 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag LMH79_RS26640 Protein ID WP_000151456.1
Coordinates 5393..5635 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS26600 (LMH79_26625) 188..490 - 303 WP_001096511.1 hypothetical protein -
LMH79_RS26605 (LMH79_26630) 580..1239 - 660 WP_000516232.1 division plane positioning ATPase MipZ -
LMH79_RS26610 (LMH79_26635) 1792..2115 + 324 WP_000432873.1 hypothetical protein -
LMH79_RS26615 (LMH79_26640) 2469..2813 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
LMH79_RS26620 (LMH79_26645) 2800..3150 + 351 WP_000127482.1 addiction module antitoxin -
LMH79_RS26625 (LMH79_26650) 3984..4499 + 516 WP_001025393.1 J domain-containing protein -
LMH79_RS26630 (LMH79_26655) 4603..5145 + 543 WP_001083904.1 hypothetical protein -
LMH79_RS26635 (LMH79_26660) 5142..5396 + 255 WP_001230709.1 hypothetical protein Toxin
LMH79_RS26640 (LMH79_26665) 5393..5635 + 243 WP_000151456.1 hypothetical protein Antitoxin
LMH79_RS26645 (LMH79_26670) 5908..6090 + 183 WP_001744154.1 hypothetical protein -
LMH79_RS26650 (LMH79_26675) 6206..6499 + 294 WP_000673431.1 hypothetical protein -
LMH79_RS26655 (LMH79_26680) 6804..7091 + 288 WP_000606467.1 hypothetical protein -
LMH79_RS26660 (LMH79_26685) 7519..7752 + 234 WP_000093745.1 hypothetical protein -
LMH79_RS26665 (LMH79_26690) 7828..8130 + 303 WP_106504554.1 CcgAII protein -
LMH79_RS26670 (LMH79_26695) 8402..8551 + 150 WP_000903193.1 hypothetical protein -
LMH79_RS26675 (LMH79_26700) 8662..9483 + 822 WP_032283064.1 DUF932 domain-containing protein -
LMH79_RS26680 (LMH79_26705) 9780..10427 - 648 WP_000614287.1 transglycosylase SLT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..51013 51013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T222881 WP_001230709.1 NZ_CP086262:5142-5396 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T222881 NZ_CP118149:6590510-6590692 [Pseudomonas chlororaphis]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT222881 WP_000151456.1 NZ_CP086262:5393-5635 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT222881 NZ_CP118149:6590725-6591150 [Pseudomonas chlororaphis]
ATGTTCAAGTACGCATTGGAAATTCACGAAGAGCCCGGCACCGTCTGGCTTTCGTGTGAGGAGATTCCAGAATTTCACGC
TGCCGGCGACACCGTAGGCGAGGCGCTGGATAGTGTTTTGGATGCGCTCGAAACGGCTCTGTCTATCTATGTCGATGAAC
GCCGCCCGATTCCATCTGGTAACGCCGAGGCTCAGGCAGGGCACGTTCTGTTATGCCTTCCAGCCCTCACCGCGGCGAAG
GTTGCACTGTGGAACGCGGTGTTGGATGAAGGCACCACCAAGGCAGAGCTGGCGCGCCGCCTGGGGATTCAGCGGCCGCA
GGTGGACCGCTTGCTGGACTTCCTGCACGACTCGAAAATCGAAAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References