Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 36807..37366 | Replicon | plasmid pB-9427-2 |
| Accession | NZ_CP086261 | ||
| Organism | Escherichia coli strain SCPM-O-B-9427 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | LMH79_RS26400 | Protein ID | WP_000421273.1 |
| Coordinates | 36807..37082 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | LMH79_RS26405 | Protein ID | WP_001387467.1 |
| Coordinates | 37082..37366 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH79_RS26355 (LMH79_26380) | 31849..32079 | + | 231 | WP_001261288.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LMH79_RS26360 (LMH79_26385) | 32103..32504 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| LMH79_RS26365 (LMH79_26390) | 32587..32742 | - | 156 | WP_000332512.1 | transposase domain-containing protein | - |
| LMH79_RS26370 (LMH79_26395) | 33081..33518 | + | 438 | WP_032159536.1 | hypothetical protein | - |
| LMH79_RS26375 (LMH79_26400) | 33828..34805 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| LMH79_RS26380 (LMH79_26405) | 35084..35824 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| LMH79_RS26385 (LMH79_26410) | 35945..36100 | - | 156 | WP_044713270.1 | hypothetical protein | - |
| LMH79_RS26390 (LMH79_26415) | 36193..36483 | - | 291 | Protein_53 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LMH79_RS26395 (LMH79_26420) | 36470..36738 | - | 269 | Protein_54 | type II toxin-antitoxin system ParD family antitoxin | - |
| LMH79_RS26400 (LMH79_26425) | 36807..37082 | - | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LMH79_RS26405 (LMH79_26430) | 37082..37366 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| LMH79_RS26410 (LMH79_26435) | 38274..39102 | - | 829 | Protein_57 | incFII family plasmid replication initiator RepA | - |
| LMH79_RS26415 (LMH79_26440) | 39095..39169 | - | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| LMH79_RS26420 (LMH79_26445) | 39169..39300 | - | 132 | Protein_59 | protein CopA/IncA | - |
| LMH79_RS26425 (LMH79_26450) | 39407..39661 | - | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| LMH79_RS26430 (LMH79_26455) | 39942..40922 | + | 981 | WP_000019440.1 | IS5-like element ISKpn26 family transposase | - |
| LMH79_RS26435 (LMH79_26460) | 41373..41585 | - | 213 | Protein_62 | ANR family transcriptional regulator | - |
| LMH79_RS26440 (LMH79_26465) | 41650..42327 | + | 678 | WP_001339397.1 | IS66-like element accessory protein TnpA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aap/aspU / aggA / aggB / aggC / aggD | 1..75544 | 75544 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T222880 WP_000421273.1 NZ_CP086261:c37082-36807 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T222880 NZ_CP086261:c37082-36807 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT222880 WP_001387467.1 NZ_CP086261:c37366-37082 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT222880 NZ_CP086261:c37366-37082 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |