Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 30729..31254 | Replicon | plasmid pB-9427-2 |
| Accession | NZ_CP086261 | ||
| Organism | Escherichia coli strain SCPM-O-B-9427 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | A0A0E0Y871 |
| Locus tag | LMH79_RS26345 | Protein ID | WP_001159872.1 |
| Coordinates | 30729..31034 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | A0A0E0Y6P9 |
| Locus tag | LMH79_RS26350 | Protein ID | WP_000813638.1 |
| Coordinates | 31036..31254 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH79_RS26320 (LMH79_26345) | 26901..27317 | - | 417 | WP_001278815.1 | plasmid partitioning/stability family protein | - |
| LMH79_RS26325 (LMH79_26350) | 27310..28290 | - | 981 | WP_000688504.1 | plasmid segregation protein ParM | - |
| LMH79_RS26330 (LMH79_26355) | 28704..29012 | - | 309 | WP_000030204.1 | molecular chaperone GroEL | - |
| LMH79_RS26335 (LMH79_26360) | 29099..29743 | - | 645 | WP_001144032.1 | ParA family protein | - |
| LMH79_RS26340 (LMH79_26365) | 29922..30728 | - | 807 | WP_000016961.1 | site-specific integrase | - |
| LMH79_RS26345 (LMH79_26370) | 30729..31034 | - | 306 | WP_001159872.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| LMH79_RS26350 (LMH79_26375) | 31036..31254 | - | 219 | WP_000813638.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| LMH79_RS26355 (LMH79_26380) | 31849..32079 | + | 231 | WP_001261288.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LMH79_RS26360 (LMH79_26385) | 32103..32504 | + | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| LMH79_RS26365 (LMH79_26390) | 32587..32742 | - | 156 | WP_000332512.1 | transposase domain-containing protein | - |
| LMH79_RS26370 (LMH79_26395) | 33081..33518 | + | 438 | WP_032159536.1 | hypothetical protein | - |
| LMH79_RS26375 (LMH79_26400) | 33828..34805 | + | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| LMH79_RS26380 (LMH79_26405) | 35084..35824 | - | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| LMH79_RS26385 (LMH79_26410) | 35945..36100 | - | 156 | WP_044713270.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aap/aspU / aggA / aggB / aggC / aggD | 1..75544 | 75544 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11722.51 Da Isoelectric Point: 5.6919
>T222878 WP_001159872.1 NZ_CP086261:c31034-30729 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI
Download Length: 306 bp
>T222878 NZ_CP118149:4672341-4672691 [Pseudomonas chlororaphis]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8344.29 Da Isoelectric Point: 4.5203
>AT222878 WP_000813638.1 NZ_CP086261:c31254-31036 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW
Download Length: 219 bp
>AT222878 NZ_CP118149:4672681-4672995 [Pseudomonas chlororaphis]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCATCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCATCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y871 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6P9 |