Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 71315..71874 Replicon plasmid pB-9427-1
Accession NZ_CP086260
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag LMH79_RS26065 Protein ID WP_000421257.1
Coordinates 71315..71590 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag LMH79_RS26070 Protein ID WP_001178089.1
Coordinates 71590..71874 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS26035 (LMH79_26060) 66475..67101 + 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -
LMH79_RS26040 (LMH79_26065) 67098..67412 + 315 WP_228355606.1 ribbon-helix-helix domain-containing protein -
LMH79_RS26045 (LMH79_26070) 67931..69277 - 1347 WP_021537814.1 protein YagA -
LMH79_RS26050 (LMH79_26075) 69631..70233 + 603 WP_228355607.1 ProQ/FINO family protein -
LMH79_RS26055 (LMH79_26080) 70471..70737 + 267 WP_000627831.1 hypothetical protein -
LMH79_RS26060 (LMH79_26085) 71006..71209 - 204 Protein_81 hypothetical protein -
LMH79_RS26065 (LMH79_26090) 71315..71590 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMH79_RS26070 (LMH79_26095) 71590..71874 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
LMH79_RS26075 (LMH79_26100) 72782..73813 - 1032 WP_000907880.1 plasmid replication initiator RepA -
LMH79_RS26080 (LMH79_26105) 74649..75155 + 507 WP_001335626.1 transcription termination/antitermination NusG family protein -
LMH79_RS26085 (LMH79_26110) 75296..75958 + 663 WP_000154142.1 hypothetical protein -
LMH79_RS26090 (LMH79_26115) 76225..76845 + 621 WP_000176497.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..83340 83340


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T222877 WP_000421257.1 NZ_CP086260:c71590-71315 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T222877 NZ_CP118149:c2598975-2598808 [Pseudomonas chlororaphis]
TTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTTCAAGCATCCCTACCG
GCCGGAGACCGTTCCAGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCCAGAAACTGGCCGGGC
TGATATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT222877 WP_001178089.1 NZ_CP086260:c71874-71590 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT222877 NZ_CP118149:c2598772-2598371 [Pseudomonas chlororaphis]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAGCTGGCCCGCG
AGGGGCAAACCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGTTATGTGATTCAGCGGAT
TGATCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References