Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4788439..4788936 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LMH79_RS23450 Protein ID WP_001173343.1
Coordinates 4788439..4788612 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LMH79_RS23455 Protein ID WP_000398619.1
Coordinates 4788640..4788936 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS23440 (4785109) 4785109..4786566 + 1458 WP_000856837.1 dipeptide/tripeptide permease DtpC -
LMH79_RS23445 (4786803) 4786803..4788320 + 1518 WP_001295074.1 lysine--tRNA ligase -
LMH79_RS23450 (4788439) 4788439..4788612 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LMH79_RS23455 (4788640) 4788640..4788936 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LMH79_RS23460 (4789163) 4789163..4789435 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LMH79_RS23465 (4789447) 4789447..4789677 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LMH79_RS23470 (4789858) 4789858..4791489 + 1632 WP_001216486.1 sensor histidine kinase -
LMH79_RS23475 (4791486) 4791486..4792205 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T222875 WP_001173343.1 NZ_CP086259:c4788612-4788439 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T222875 NZ_CP118149:544155-544337 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAATA
CAAGCATCTGCACAAGCCGGGACGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT222875 WP_000398619.1 NZ_CP086259:c4788936-4788640 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT222875 NZ_CP118149:544367-544768 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTATGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGCTTTCTCGCGGCAGCGGCATTGCGCGAACTCTCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References