Detailed information of TA system TA222870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3921807..3922644
• Replicon: chromosome
• Accession: NZ_CP086259
• Organism: Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LMH79_RS19295
• Protein ID: WP_000227784.1
• Coordinates: 3922102..3922644 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LMH79_RS19290
• Protein ID: WP_001297137.1
• Coordinates: 3921807..3922118 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH79_RS19265 (3916827)	3916827..3917774	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LMH79_RS19270 (3917796)	3917796..3919787	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LMH79_RS19275 (3919777)	3919777..3920391	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LMH79_RS19280 (3920391)	3920391..3920720	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LMH79_RS19285 (3920732)	3920732..3921622	+	891	WP_000971336.1	heme o synthase	-
LMH79_RS19290 (3921807)	3921807..3922118	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LMH79_RS19295 (3922102)	3922102..3922644	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LMH79_RS19300 (3922700)	3922700..3923635	-	936	WP_024174603.1	tetratricopeptide repeat protein	-
LMH79_RS19305 (3924043)	3924043..3925407	+	1365	WP_001000974.1	MFS transporter	-
LMH79_RS19310 (3925535)	3925535..3926026	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LMH79_RS19315 (3926194)	3926194..3927105	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T222870 WP_000227784.1 NZ_CP086259:3922102-3922644 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T222870 NZ_CP118148:4633287-4633637 [Pseudomonas chlororaphis]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT222870 WP_001297137.1 NZ_CP086259:3921807-3922118 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT222870 NZ_CP118148:4633627-4633941 [Pseudomonas chlororaphis]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCATCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3