Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3887730..3888348 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LMH79_RS19125 Protein ID WP_001291435.1
Coordinates 3888130..3888348 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LMH79_RS19120 Protein ID WP_000344800.1
Coordinates 3887730..3888104 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS19110 (3882819) 3882819..3884012 + 1194 WP_228355561.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH79_RS19115 (3884035) 3884035..3887184 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LMH79_RS19120 (3887730) 3887730..3888104 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LMH79_RS19125 (3888130) 3888130..3888348 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LMH79_RS19130 (3888519) 3888519..3889070 + 552 WP_000102564.1 maltose O-acetyltransferase -
LMH79_RS19135 (3889186) 3889186..3889656 + 471 WP_000136192.1 YlaC family protein -
LMH79_RS19140 (3889820) 3889820..3891370 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LMH79_RS19145 (3891412) 3891412..3891765 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LMH79_RS19155 (3892144) 3892144..3892455 + 312 WP_000409911.1 MGMT family protein -
LMH79_RS19160 (3892486) 3892486..3893058 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222869 WP_001291435.1 NZ_CP086259:3888130-3888348 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T222869 NZ_CP118148:c2509262-2508969 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCGTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT222869 WP_000344800.1 NZ_CP086259:3887730-3888104 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT222869 NZ_CP118148:c2508967-2508677 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGCCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References