Detailed information of TA system TA222859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2512071..2513659
• Replicon: chromosome
• Accession: NZ_CP086259
• Organism: Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0E0XZ87
• Locus tag: LMH79_RS12175
• Protein ID: WP_001125467.1
• Coordinates: 2512071..2513393 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LMH79_RS12180
• Protein ID: WP_001296726.1
• Coordinates: 2513393..2513659 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH79_RS12160 (2509511)	2509511..2510221	-	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
LMH79_RS12165 (2510555)	2510555..2511118	-	564	WP_001351339.1	type 1 fimbrial major subunit FimA	-
LMH79_RS12170 (2511551)	2511551..2511759	-	209	Protein_2389	AraC family transcriptional regulator	-
LMH79_RS12175 (2512071)	2512071..2513393	-	1323	WP_001125467.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LMH79_RS12180 (2513393)	2513393..2513659	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49395.03 Da
• Isoelectric Point: 9.1203

>T222859 WP_001125467.1 NZ_CP086259:c2513393-2512071 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLSVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T222859 NZ_CP118147:1232654-1232932 [Pseudomonas chlororaphis]
GTGAGGGAATTGAAATGGACGAGCAAGGCACTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCAGCTCGAACTGTGCAATCGCTCACGCAGGCCCCGGATACCCTGCTGACCAATCCGCGTATCGGCGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCAGGATGCGACGCTC
TACATCCTGCGGGTGTGGCATGTACGAGGAGACCGCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT222859 WP_001296726.1 NZ_CP086259:c2513659-2513393 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT222859 NZ_CP118147:1232394-1232657 [Pseudomonas chlororaphis]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCCCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTGGCGTGGGTCGATCAGGAAGAGGAGCGCAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGCTGGGCGGGTCATCGATCACCAGGCGGTCCAGGCATGGGCCGAGAGCCTGATGAATGAC
AAGCCTTTGCCGGTGCCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XZ87