Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1472542..1473167 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LMH79_RS07140 Protein ID WP_000911329.1
Coordinates 1472769..1473167 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LMH79_RS07135 Protein ID WP_000450524.1
Coordinates 1472542..1472769 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS07110 (1468344) 1468344..1468814 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
LMH79_RS07115 (1468814) 1468814..1469386 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LMH79_RS07120 (1469532) 1469532..1470410 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LMH79_RS07125 (1470427) 1470427..1471461 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LMH79_RS07130 (1471674) 1471674..1472387 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LMH79_RS07135 (1472542) 1472542..1472769 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LMH79_RS07140 (1472769) 1472769..1473167 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH79_RS07145 (1473314) 1473314..1474177 + 864 WP_001267498.1 neutral zinc metallopeptidase -
LMH79_RS07150 (1474192) 1474192..1476207 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
LMH79_RS07155 (1476281) 1476281..1476979 + 699 WP_000679823.1 esterase -
LMH79_RS07160 (1477089) 1477089..1477289 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T222856 WP_000911329.1 NZ_CP086259:1472769-1473167 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T222856 NZ_CP118147:563616-563798 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT222856 WP_000450524.1 NZ_CP086259:1472542-1472769 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT222856 NZ_CP118147:563828-564229 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATT
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTACGCGAGTTATCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References