Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1027632..1028286 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LMH79_RS05040 Protein ID WP_000244772.1
Coordinates 1027879..1028286 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LMH79_RS05035 Protein ID WP_000354046.1
Coordinates 1027632..1027898 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS05010 (1022919) 1022919..1023662 + 744 WP_000951964.1 SDR family oxidoreductase -
LMH79_RS05015 (1023719) 1023719..1025152 - 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
LMH79_RS05020 (1025197) 1025197..1025508 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LMH79_RS05025 (1025672) 1025672..1026331 + 660 WP_000250274.1 hemolysin III family protein -
LMH79_RS05030 (1026409) 1026409..1027389 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LMH79_RS05035 (1027632) 1027632..1027898 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LMH79_RS05040 (1027879) 1027879..1028286 + 408 WP_000244772.1 protein YgfX Toxin
LMH79_RS05045 (1028326) 1028326..1028847 - 522 WP_001055874.1 flavodoxin FldB -
LMH79_RS05050 (1028959) 1028959..1029855 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LMH79_RS05055 (1029880) 1029880..1030590 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LMH79_RS05060 (1030596) 1030596..1032329 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T222854 WP_000244772.1 NZ_CP086259:1027879-1028286 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T222854 NZ_CP118146:c6112802-6112503 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCGCCGGTGGGCGAAGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATTCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGGGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT222854 WP_000354046.1 NZ_CP086259:1027632-1027898 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT222854 NZ_CP118146:c6112506-6112213 [Pseudomonas chlororaphis]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCCTGCGCGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGTATGACCGAACTGGCCC
ATGAAACCGGGCTGTCTCGCGAGCAGCTGTACCGCTCATTCAGCGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGGCTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References