Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 354867..355453 Replicon chromosome
Accession NZ_CP086259
Organism Escherichia coli strain SCPM-O-B-9427

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LMH79_RS01640 Protein ID WP_042014162.1
Coordinates 354867..355097 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LMH79_RS01645 Protein ID WP_000593555.1
Coordinates 355094..355453 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH79_RS01630 (350904) 350904..353639 + 2736 WP_000149085.1 ribosome-associated ATPase/putative transporter RbbA -
LMH79_RS01635 (353639) 353639..354763 + 1125 WP_001216257.1 ABC transporter permease -
LMH79_RS01640 (354867) 354867..355097 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LMH79_RS01645 (355094) 355094..355453 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LMH79_RS01650 (355573) 355573..355974 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LMH79_RS01655 (355980) 355980..356786 - 807 WP_000173630.1 nickel import ATP-binding protein NikE -
LMH79_RS01660 (356783) 356783..357547 - 765 WP_001136229.1 nickel import ATP-binding protein NikD -
LMH79_RS01665 (357547) 357547..358380 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LMH79_RS01670 (358377) 358377..359321 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T222851 WP_042014162.1 NZ_CP086259:354867-355097 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T222851 NZ_CP118146:c2551758-2551468 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCTCGCGGCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT222851 WP_000593555.1 NZ_CP086259:355094-355453 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT222851 NZ_CP118146:c2552053-2551760 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGCCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTGCGCGGCCACACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References