Detailed information of TA system TA222847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2348735..2349651
• Replicon: chromosome
• Accession: NZ_CP086257
• Organism: Providencia rettgeri strain KM4

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LL668_RS10525
• Protein ID: WP_196718396.1
• Coordinates: 2349193..2349651 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6G6PJY5
• Locus tag: LL668_RS10520
• Protein ID: WP_004910826.1
• Coordinates: 2348735..2349196 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL668_RS10490 (LL668_10500)	2344169..2344642	-	474	WP_109912202.1	NUDIX domain-containing protein	-
LL668_RS10495 (LL668_10505)	2344935..2345576	-	642	WP_004910832.1	leucine efflux protein LeuE	-
LL668_RS10500 (LL668_10510)	2346062..2346916	+	855	WP_004910830.1	AraC family transcriptional regulator	-
LL668_RS10505 (LL668_10515)	2346975..2347676	+	702	WP_123381951.1	AzlC family ABC transporter permease	-
LL668_RS10510 (LL668_10520)	2347673..2347987	+	315	WP_004910828.1	AzlD domain-containing protein	-
LL668_RS10515 (LL668_10525)	2348179..2348646	+	468	WP_109912201.1	DUF1456 family protein	-
LL668_RS10520 (LL668_10530)	2348735..2349196	+	462	WP_004910826.1	DUF2384 domain-containing protein	Antitoxin
LL668_RS10525 (LL668_10535)	2349193..2349651	+	459	WP_196718396.1	RES domain-containing protein	Toxin
LL668_RS10530 (LL668_10540)	2349652..2350542	-	891	WP_228366983.1	LysR family transcriptional regulator	-
LL668_RS10535 (LL668_10545)	2350588..2351547	-	960	WP_109912198.1	acetyl-CoA carboxylase carboxyl transferase subunit alpha	-
LL668_RS10540 (LL668_10550)	2351653..2352075	-	423	WP_004910820.1	helix-turn-helix domain-containing protein	-
LL668_RS10545 (LL668_10555)	2352106..2352633	-	528	WP_228366984.1	GNAT family N-acetyltransferase	-
LL668_RS10550 (LL668_10560)	2352650..2354116	-	1467	WP_042851594.1	AMP nucleosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-146)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (99-150)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17174.75 Da
• Isoelectric Point: 6.2048

>T222847 WP_196718396.1 NZ_CP086257:2349193-2349651 [Providencia rettgeri]
MILYRLVKSSFVHDAWSGQGAMLYGGRWNHKGTPAVYTSTSISLATLEILVHINQDLLLNQFSLLSIDINDRYVMKLSLD
HLPTDWQQDPAPTSTMDIGTTWLNNQDSLVLLIPSCIIPYEYNAIINPLHPQFQKAIHTVKPLDFSVDPRLA

Nucleotide

• Length: 459 bp

>T222847 NZ_CP118146:563402-563584 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17060.54 Da
• Isoelectric Point: 9.9140

>AT222847 WP_004910826.1 NZ_CP086257:2348735-2349196 [Providencia rettgeri]
MRNYIPTTPLQPTASSFKPLWRHVGLPAERGIELTHFLRQGLSVSVLDNIQEWSGMSKTELLRISGINERNIARRKNAGQ
PLNADESERIARLVRVFDAAVRLFNGDKHAAGDWLNHPVKGLGHLRPIELIATESGAIEVIDLIGRIEHGVFS

Nucleotide

• Length: 462 bp

>AT222847 NZ_CP118146:563614-564015 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATT
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTACGCGAGTTATCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A6G6PJY5