Detailed information of TA system TA222842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 193299..193819
• Replicon: chromosome
• Accession: NZ_CP086254
• Organism: Brachybacterium halotolerans subsp. kimchii strain CBA3105

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: Bra3105_RS00880
• Protein ID: WP_200500941.1
• Coordinates: 193529..193819 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: Bra3105_RS00875
• Protein ID: WP_200500942.1
• Coordinates: 193299..193532 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra3105_RS00850 (Bra3105_00855)	188550..188735	-	186	WP_228358219.1	hypothetical protein	-
Bra3105_RS00855 (Bra3105_00860)	188868..189716	+	849	WP_228358220.1	N-acetyltransferase	-
Bra3105_RS00860 (Bra3105_00865)	189790..190086	+	297	WP_152352722.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
Bra3105_RS00865 (Bra3105_00870)	190089..191642	+	1554	WP_228358221.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
Bra3105_RS00870 (Bra3105_00875)	191642..193153	+	1512	WP_200500943.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
Bra3105_RS00875 (Bra3105_00880)	193299..193532	+	234	WP_200500942.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
Bra3105_RS00880 (Bra3105_00885)	193529..193819	+	291	WP_200500941.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Bra3105_RS00885 (Bra3105_00890)	193902..194435	+	534	WP_228358222.1	DUF664 domain-containing protein	-
Bra3105_RS00890 (Bra3105_00895)	194603..195595	-	993	WP_228358223.1	hypothetical protein	-
Bra3105_RS00895 (Bra3105_00900)	195716..196219	-	504	WP_228358224.1	hypothetical protein	-
Bra3105_RS00900 (Bra3105_00905)	196392..198455	+	2064	WP_228358225.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Antitoxin ParD (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11410.84 Da
• Isoelectric Point: 9.5837

>T222842 WP_200500941.1 NZ_CP086254:193529-193819 [Brachybacterium halotolerans subsp. kimchii]
MKRFRLTPAAQRDLSEIWDFTEDRWSASQAESYLRDIQNAIERLAADPDRGRPVDDIRQGYRRYGIGSHLVFYVQRGDRI
DVIRILHQRMDPGRHL

Nucleotide

• Length: 291 bp

>T222842 NZ_CP118145:c1771887-1771705 [Pseudomonas chlororaphis]
GTGGACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGGGGCAGTCACCACCATTT
CAAGCATCCCCATAAACCGGGGCTGGTGACCGTTCCCCATCCCAAGAAGGACCTGCTGAGAAAAACCGCCATCAGTATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8421.27 Da
• Isoelectric Point: 4.2550

>AT222842 WP_200500942.1 NZ_CP086254:193299-193532 [Brachybacterium halotolerans subsp. kimchii]
MAQNTSISLDDHYSGFLAEQVASGRYRSSSEVIRAGLRLLEDRETQLAALREALVVGEESGEAQPFDFDAFIASKQA

Nucleotide

• Length: 234 bp

>AT222842 NZ_CP118145:c1771672-1771265 [Pseudomonas chlororaphis]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGGGTGGAAGTCCCCGATATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCTGAAG
ACGGTGCCCCCATCCCGCACGCCAACAAGGTCACCCTGCACGCGGCCAACCCCCGGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACTTGCTGAGCCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCCAAGAGCCGCTCGGGTTTTCTCGCTTCGGCTGCACTGAAGGTATTGCAAC
AAGACTGA