Detailed information of TA system TA222841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4458139..4459724
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: DMB82_RS20635
• Protein ID: WP_116164524.1
• Coordinates: 4458405..4459724 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: DMB82_RS20630
• Protein ID: WP_102117970.1
• Coordinates: 4458139..4458405 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS20610 (DMB82_0020670)	4454341..4455843	-	1503	WP_116164525.1	MFS transporter	-
DMB82_RS20615 (DMB82_0020675)	4455947..4456501	+	555	WP_102117973.1	TetR/AcrR family transcriptional regulator	-
DMB82_RS20620 (DMB82_0020680)	4456551..4456961	-	411	WP_102117972.1	helix-turn-helix domain-containing protein	-
DMB82_RS20625 (DMB82_0020685)	4457104..4457955	+	852	WP_102117971.1	SDR family oxidoreductase	-
DMB82_RS20630 (DMB82_0020690)	4458139..4458405	+	267	WP_102117970.1	helix-turn-helix domain-containing protein	Antitoxin
DMB82_RS20635 (DMB82_0020695)	4458405..4459724	+	1320	WP_116164524.1	type II toxin-antitoxin system HipA family toxin	Toxin
DMB82_RS20640 (DMB82_0020700)	4459881..4461245	-	1365	WP_116156053.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
DMB82_RS20645 (DMB82_0020705)	4461374..4463008	-	1635	WP_102117967.1	membrane protein insertase YidC	-
DMB82_RS20650 (DMB82_0020710)	4463011..4463268	-	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
DMB82_RS20655 (DMB82_0020715)	4463232..4463591	-	360	WP_010302382.1	ribonuclease P protein component	-
DMB82_RS20660 (DMB82_0020720)	4463610..4463750	-	141	WP_005976668.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49218.42 Da
• Isoelectric Point: 6.9025

>T222841 WP_116164524.1 NZ_CP086253:4458405-4459724 [Pectobacterium aquaticum]
MAALDVYMNGYRVGILTKTGSGAHHFSYDANWLGLPGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
ADSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIKYKTLSEQQLENILAGYLSDAPLGMIDTEDNFRISIAGAQEKTA
LLYLDDHWCLPLNATPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSADPERDRYRFMQAQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDHREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAKAVGFARESMENILVEFSNTV
ETVMTTVGSQLPVDFPTHISDAILTGLQARSRRLMRGWE

Nucleotide

• Length: 1320 bp

>T222841 NZ_CP118145:1215347-1215625 [Pseudomonas chlororaphis]
GTGAGGGAATTGAAATGGACGAGCAAGGCACTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCAGCTCGAACGGTGCAATCGCTCACGCAGGCCCCGGATACCCTGCTGACCAATCCGCGTATCGGCGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCAGGATGCGACGCTC
TACATCCTGCGGGTGTGGCATGTACGAGAAGACCGCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9886.17 Da
• Isoelectric Point: 10.0982

>AT222841 WP_102117970.1 NZ_CP086253:4458139-4458405 [Pectobacterium aquaticum]
MKVTNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVNTANNEASV
ASSWKEEW

Nucleotide

• Length: 267 bp

>AT222841 NZ_CP118145:1215087-1215350 [Pseudomonas chlororaphis]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCCCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTGGCGTGGGTCGATCAGGAAGAGGAGCGCAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGCTGGGCGGGTCATCGATCACCAGGCTGTCCAGGCATGGGCCGAGAGCCTGATGAATGAC
AAGCCTTTGCCGGTGCCCAAGTGA