Detailed information of TA system TA222836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3418785..3419821
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: DMB82_RS15860
• Protein ID: WP_116156327.1
• Coordinates: 3418785..3419360 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A6P1RQC6
• Locus tag: DMB82_RS15865
• Protein ID: WP_039497370.1
• Coordinates: 3419360..3419821 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS15840 (DMB82_0015890)	3416652..3417044	-	393	WP_116163976.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DMB82_RS15845 (DMB82_0015895)	3417031..3417486	-	456	WP_228400003.1	YgdB family protein	-
DMB82_RS15850 (DMB82_0015900)	3417483..3418109	-	627	WP_116163980.1	prepilin peptidase-dependent protein	-
DMB82_RS15855 (DMB82_0015905)	3418103..3418606	-	504	WP_116163982.1	prepilin peptidase-dependent protein	-
DMB82_RS15860 (DMB82_0015910)	3418785..3419360	-	576	WP_116156327.1	PIN domain-containing protein	Toxin
DMB82_RS15865 (DMB82_0015915)	3419360..3419821	-	462	WP_039497370.1	helix-turn-helix domain-containing protein	Antitoxin
DMB82_RS15870 (DMB82_0015920)	3420045..3420839	-	795	WP_039312476.1	thymidylate synthase	-
DMB82_RS15875 (DMB82_0015925)	3420876..3421742	-	867	WP_116164006.1	prolipoprotein diacylglyceryl transferase	-
DMB82_RS15880 (DMB82_0015930)	3421969..3424215	-	2247	WP_102118308.1	phosphoenolpyruvate--protein phosphotransferase	-
DMB82_RS15885 (DMB82_0015935)	3424228..3424761	-	534	WP_116163984.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21545.95 Da
• Isoelectric Point: 5.0130

>T222836 WP_116156327.1 NZ_CP086253:c3419360-3418785 [Pectobacterium aquaticum]
MNHSPYPVVLDACVIYPSLLRDLLMHLGLTGLYQPKWTVKIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGY
EQLISALVLPDVGDRHVLAAAIRANAEVIVTANLKDFPATALFEFGVEAMHPDDFIADLFDLNSALVLKAVRTSRTAFRK
PPFDVDEYLDCLQRQGLPMTVKELDAYRSLI

Nucleotide

• Length: 576 bp

>T222836 NZ_CP118144:c6352009-6351716 [Pseudomonas chlororaphis]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCCTGCGCGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCATTCAGCGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGGCTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17243.76 Da
• Isoelectric Point: 6.2253

>AT222836 WP_039497370.1 NZ_CP086253:c3419821-3419360 [Pectobacterium aquaticum]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDAHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQELGFY

Nucleotide

• Length: 462 bp

>AT222836 NZ_CP118144:c6352305-6352006 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCGCCGGTGGGCGAAGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGGGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1RQC6