Detailed information of TA system TA222834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3225576..3226201
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: DMB82_RS14980
• Protein ID: WP_005976087.1
• Coordinates: 3225998..3226201 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: DMB82_RS14975
• Protein ID: WP_102116758.1
• Coordinates: 3225576..3225944 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS14960 (DMB82_0015010)	3221206..3224334	+	3129	WP_102116756.1	efflux RND transporter permease subunit	-
DMB82_RS14965 (DMB82_0015015)	3224569..3224817	+	249	WP_102116757.1	type B 50S ribosomal protein L31	-
DMB82_RS14970 (DMB82_0015020)	3224835..3224978	+	144	WP_005976091.1	type B 50S ribosomal protein L36	-
DMB82_RS14975 (DMB82_0015025)	3225576..3225944	+	369	WP_102116758.1	Hha toxicity modulator TomB	Antitoxin
DMB82_RS14980 (DMB82_0015030)	3225998..3226201	+	204	WP_005976087.1	HHA domain-containing protein	Toxin
DMB82_RS14990 (DMB82_0015040)	3226945..3227265	+	321	WP_010282239.1	MGMT family protein	-
DMB82_RS14995 (DMB82_0015045)	3227285..3227866	-	582	WP_116155836.1	YbaY family lipoprotein	-
DMB82_RS15000 (DMB82_0015050)	3228077..3228940	+	864	WP_102116760.1	acyl-CoA thioesterase II	-
DMB82_RS15005 (DMB82_0015055)	3229126..3230412	-	1287	WP_167469165.1	ammonium transporter AmtB	-
DMB82_RS15010 (DMB82_0015060)	3230452..3230790	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T222834 WP_005976087.1 NZ_CP086253:3225998-3226201 [Pectobacterium aquaticum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T222834 NZ_CP118144:c2598949-2598659 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGTGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGCGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14102.92 Da
• Isoelectric Point: 4.3697

>AT222834 WP_102116758.1 NZ_CP086253:3225576-3225944 [Pectobacterium aquaticum]
MDEYTPKYYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINIQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICMPAKT

Nucleotide

• Length: 369 bp

>AT222834 NZ_CP118144:c2599244-2598951 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATATGGGAGCCGGCTACCGCATCTATTTCACCGTGCGCGGTAATACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0