Detailed information of TA system TA222832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1688524..1689182
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DMB82_RS07895
• Protein ID: WP_116162549.1
• Coordinates: 1688754..1689182 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DMB82_RS07890
• Protein ID: WP_116162551.1
• Coordinates: 1688524..1688754 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS07850 (DMB82_0007890)	1684226..1684591	+	366	WP_116162567.1	hypothetical protein	-
DMB82_RS07855 (DMB82_0007895)	1684634..1685041	+	408	WP_116162565.1	hypothetical protein	-
DMB82_RS07860 (DMB82_0007900)	1685074..1685286	+	213	WP_116162563.1	hypothetical protein	-
DMB82_RS07865 (DMB82_0007905)	1685368..1686189	+	822	WP_116162561.1	DUF932 domain-containing protein	-
DMB82_RS07870 (DMB82_0007910)	1686242..1686715	+	474	WP_116162559.1	DNA repair protein RadC	-
DMB82_RS07875 (DMB82_0007915)	1686755..1687090	+	336	WP_116162557.1	type IV toxin-antitoxin system YeeU family antitoxin	-
DMB82_RS07880 (DMB82_0007920)	1687160..1687480	+	321	WP_116162555.1	TA system toxin CbtA family protein	-
DMB82_RS07885 (DMB82_0007925)	1687596..1688429	+	834	WP_116162553.1	DUF4942 domain-containing protein	-
DMB82_RS07890 (DMB82_0007930)	1688524..1688754	+	231	WP_116162551.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DMB82_RS07895 (DMB82_0007935)	1688754..1689182	+	429	WP_116162549.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DMB82_RS07900 (DMB82_0007940)	1689493..1689690	-	198	WP_226888919.1	hypothetical protein	-
DMB82_RS07905 (DMB82_0007945)	1689894..1690181	-	288	WP_102118418.1	HigA family addiction module antitoxin	-
DMB82_RS07910 (DMB82_0007950)	1690181..1690459	-	279	WP_102118419.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DMB82_RS07915 (DMB82_0007955)	1690589..1690969	-	381	WP_208644314.1	hypothetical protein	-
DMB82_RS07920 (DMB82_0007960)	1691489..1691626	+	138	WP_240348903.1	YnfU family zinc-binding protein	-
DMB82_RS07925 (DMB82_0007965)	1691665..1691856	+	192	WP_116162547.1	hypothetical protein	-
DMB82_RS07930 (DMB82_0007970)	1691944..1692867	+	924	WP_116162681.1	LpxL/LpxP family Kdo(2)-lipid IV(A) lauroyl/palmitoleoyl acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1656312..1692867	36555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15744.97 Da
• Isoelectric Point: 6.7190

>T222832 WP_116162549.1 NZ_CP086253:1688754-1689182 [Pectobacterium aquaticum]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISAITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQTYGFDAAVHT
GQIRAELAKQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGSSADNSQPL

Nucleotide

• Length: 429 bp

>T222832 NZ_CP118144:1221912-1222190 [Pseudomonas chlororaphis]
GTGAGGGAATTGAAATGGACGAGCAAGGCACTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCAGCTCGAACTGTGCACTCGCTCACGCAGGCCCCGGATACCCTGCTGATCAATCCGCGTATCGGCGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCAGGATGCGACGCTC
TACATCTTGCGGGTGTGGCATGTACGAGAAGACCGCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8681.76 Da
• Isoelectric Point: 4.4091

>AT222832 WP_116162551.1 NZ_CP086253:1688524-1688754 [Pectobacterium aquaticum]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIIAIGRTRIITPAGESWDSWFEGEGVTPDFMITREQQDDQIREDF

Nucleotide

• Length: 231 bp

>AT222832 NZ_CP118144:1221652-1221915 [Pseudomonas chlororaphis]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCCCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTAGCGTGGGTCGATCAGGAAGAGGAGCGCAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGTTGGGCGGGTCATCGATCACCAGGCTGTCCAGGCATGGGCCGAGAGCCTGATGAATGAC
AAGCCTTTGCCGGTGCCCAAGTGA