Detailed information of TA system TA222829

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 810769..811429
• Replicon: chromosome
• Accession: NZ_CP086253
• Organism: Pectobacterium aquaticum strain A212-S19-A16

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: DMB82_RS03850
• Protein ID: WP_226373417.1
• Coordinates: 811055..811429 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: DMB82_RS03845
• Protein ID: WP_005971623.1
• Coordinates: 810769..811035 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB82_RS03825 (DMB82_0003850)	806918..807922	-	1005	WP_102118033.1	LacI family DNA-binding transcriptional regulator	-
DMB82_RS03830 (DMB82_0003855)	807977..808585	-	609	WP_010301802.1	HD domain-containing protein	-
DMB82_RS03835 (DMB82_0003860)	808831..809478	+	648	WP_103183036.1	hemolysin III family protein	-
DMB82_RS03840 (DMB82_0003865)	809533..810534	-	1002	WP_102118032.1	tRNA-modifying protein YgfZ	-
DMB82_RS03845 (DMB82_0003870)	810769..811035	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
DMB82_RS03850 (DMB82_0003875)	811055..811429	+	375	WP_226373417.1	protein YgfX	Toxin
DMB82_RS03855 (DMB82_0003880)	811498..812433	+	936	WP_116164244.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
DMB82_RS03860 (DMB82_0003885)	812515..813033	-	519	WP_102118029.1	flavodoxin FldB	-
DMB82_RS03865 (DMB82_0003890)	813196..814524	-	1329	WP_014914180.1	MFS transporter	-
DMB82_RS03870 (DMB82_0003895)	814836..815735	+	900	WP_102118028.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14626.32 Da
• Isoelectric Point: 10.4737

>T222829 WP_226373417.1 NZ_CP086253:811055-811429 [Pectobacterium aquaticum]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQKNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 375 bp

>T222829 NZ_CP118144:552859-553041 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT222829 WP_005971623.1 NZ_CP086253:810769-811035 [Pectobacterium aquaticum]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT222829 NZ_CP118144:553071-553472 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGTCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTCCTTGCCGCGGCGGCATTACGCGAGTTATCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6