Detailed information of TA system TA222821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-RelB
• Location: 6080..6584
• Replicon: plasmid pCF011-68
• Accession: NZ_CP086248
• Organism: Clostridium sp. CF011

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LL036_RS18795
• Protein ID: WP_216304438.1
• Coordinates: 6327..6584 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LL036_RS18790
• Protein ID: WP_216304437.1
• Coordinates: 6080..6334 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL036_RS18760 (LL036_18695)	1605..1829	+	225	WP_216304432.1	DUF1659 domain-containing protein	-
LL036_RS18765 (LL036_18700)	1888..2124	+	237	WP_216304433.1	DUF2922 domain-containing protein	-
LL036_RS18770 (LL036_18705)	2312..3091	+	780	WP_216304434.1	N-acetylmuramoyl-L-alanine amidase	-
LL036_RS18775	3147..3272	+	126	WP_258280793.1	hypothetical protein	-
LL036_RS18780 (LL036_18710)	3336..3542	-	207	WP_216304435.1	hypothetical protein	-
LL036_RS18785 (LL036_18715)	4890..5363	-	474	WP_216304436.1	ASCH domain-containing protein	-
LL036_RS18790 (LL036_18720)	6080..6334	+	255	WP_216304437.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LL036_RS18795 (LL036_18725)	6327..6584	+	258	WP_216304438.1	Txe/YoeB family addiction module toxin	Toxin
LL036_RS18800 (LL036_18730)	7513..7746	+	234	WP_268064233.1	RepB family plasmid replication initiator protein	-
LL036_RS18805 (LL036_18735)	8000..9175	-	1176	WP_216304439.1	replication initiation protein	-
LL036_RS18810 (LL036_18740)	10085..10291	+	207	WP_216304440.1	hypothetical protein	-
LL036_RS18815 (LL036_18745)	10480..11358	+	879	WP_216304441.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68240	68240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10220.58 Da
• Isoelectric Point: 6.9776

>T222821 WP_216304438.1 NZ_CP086248:6327-6584 [Clostridium sp. CF011]
MNKIFSEVSWEHYVYWQSEDKKILRKINELIKDIERNGNEGLGKPEALKHELVGYWSRRITEVHRLIYSIDDENIYVASC
KGHYH

Nucleotide

• Length: 258 bp

>T222821 NZ_CP118143:780791-781087 [Pseudomonas chlororaphis]
ATGCGTGTAATCACGGCTAAACGAATTTGGGATGCCAAGGAAAAATGGCCACAGTCGGCCAAGGCGTTGGATGATTGGTA
TCGCGCGCTTAAACGCAATCGGCTTGCGGATTTTGCCGCGATGAAGGCGATGTTTCCGGCGGTGGACAAGGTCGGACCGC
TGCATGTGTTCGATATCGGTGGCAACAAGATACGACTGATTGCATTGGTCCGTTATACGACGCAAAAGCTCTATATCCGC
CATGTGTTGGACCACCGTGAATATGACCGGGGTAAGTGGAAGGAGGATCGAGGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9848.18 Da
• Isoelectric Point: 4.6151

>AT222821 WP_216304437.1 NZ_CP086248:6080-6334 [Clostridium sp. CF011]
MIAIGYSNVRQNFKNYCDKATKDFETIIITRERGDNVVMLSESAYNNLMENLYVRSDPTYYNELLKSIEQLKMGKGIERE
LLDE

Nucleotide

• Length: 255 bp

>AT222821 NZ_CP118143:781084-781485 [Pseudomonas chlororaphis]
ATGAGTGCATTGATCGAGCAAGTGGCCGAACACTGGCAGTTCGTTGCGCCGTTGCTGCGCAAGCCGAAAACCGAGGCGGA
CTACGACAGGCTGGTTCAGGCCCTTGACGAGTTGTTGGACCTGACAGGGGAAGATGAGGCCCATCCTCTCAACAGCCTGG
TGGACATCATCGGGGACTGGATCGAAGCCTACGATCTTGAACATCGCCCGATGCCTGTTGCCAGCGGAATAGATGTGTTG
CGCTACATGATGCGCGAACACGGTCTGACCCAGAGCGACCTGCCCGGTGTCGGCGCGCAGTCGGTAGTCTCGGAGATCCT
GAGCGGCAAGCGTCAGCTCAACCTGCGTCAGATCCGCTGGCTGGCCGAACGTTTCAAGGTCTCGATGGAAACCTTTATCT
GA