Detailed information of TA system TA222820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2424882..2425447
• Replicon: chromosome
• Accession: NZ_CP086229
• Organism: Streptomyces clavuligerus strain DSM 738

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: LL058_RS09835
• Protein ID: WP_003960643.1
• Coordinates: 2424882..2425250 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LL058_RS09840
• Protein ID: WP_029182939.1
• Coordinates: 2425250..2425447 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL058_RS09815 (LL058_09835)	2420200..2420670	+	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-
LL058_RS09820 (LL058_09840)	2420735..2421523	-	789	WP_003960641.1	S16 family serine protease	-
LL058_RS09825 (LL058_09845)	2421988..2422629	-	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
LL058_RS09830 (LL058_09850)	2422931..2424724	-	1794	WP_003954793.1	DEAD/DEAH box helicase	-
LL058_RS09835 (LL058_09855)	2424882..2425250	-	369	WP_003960643.1	Fic family protein	Toxin
LL058_RS09840 (LL058_09860)	2425250..2425447	-	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
LL058_RS09845 (LL058_09865)	2425481..2426986	-	1506	WP_003954790.1	MFS transporter	-
LL058_RS09855 (LL058_09875)	2427497..2428060	-	564	WP_003954788.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T222820 WP_003960643.1 NZ_CP086229:c2425250-2424882 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T222820 NZ_CP118143:c559965-559537 [Pseudomonas chlororaphis]
ATGATCGTGCTCGACACCAATGTGCTGTCGGAACTGATGCGTCCCCAACCGGACGCATCGGTATTGCTGTGGATCGAAGC
GCAACCTGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTTCACGGCATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGATGCTCCATGCGTCGGCCCTGGCGATGTTCGAGGAAGATTTCATCGACCGCATCGTGCCCTTCGGGACC
CAGGCGGCTCTTTACTACGCCCATCTGATTTCGCACCGGGAAAGCCTGGGGCGTCCGATCAGCCTGGTGGATGCGCAAAT
TGCGGCCATATGCCGCGTCCACGACGCGGCGATCGCCACCCGTAACGCCAAGGACTTCAGCGACACCGGGTTGATCGTTA
TCAACCCCTGGCTGCCACTCGAGGTTTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT222820 WP_029182939.1 NZ_CP086229:c2425447-2425250 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT222820 NZ_CP118143:c560207-559962 [Pseudomonas chlororaphis]
ATGGCCAGCCTGACAATCCGAAACCTGGACGACCACATCAAAGAGCGCCTGCGCATCGAGGCCGCCCACAACGGCCACTC
CATGGAAGAAGAAGCCCGCTTGATTTTGCGCCGCGCCCTGAACAGAACAACCGACAGCCAAGGCCTGGCCAGTCGTATCC
GGGCACGCTTTGCCGAGGCCGGCGGAGTGAAGCTTGAGCTGCCCGAACGTACCGAGCCGGCACGCGTGGTGGATCTGGGC
GAATGA

Structures

Toxin

• AlphaFold DB: E2Q583