Detailed information of TA system TA222818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 19538..20139
• Replicon: plasmid p779-3
• Accession: NZ_CP086223
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LMB17_RS26970
• Protein ID: WP_165467778.1
• Coordinates: 19759..20139 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A765X875
• Locus tag: LMB17_RS26965
• Protein ID: WP_032271830.1
• Coordinates: 19538..19759 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS26910 (LMB17_26920)	14710..14838	+	129	Protein_26	hypothetical protein	-
LMB17_RS26915 (LMB17_26925)	14854..15033	+	180	Protein_27	hypothetical protein	-
LMB17_RS28315 (LMB17_26930)	15301..15597	+	297	WP_224690240.1	DUF551 domain-containing protein	-
LMB17_RS26925 (LMB17_26935)	15680..15913	+	234	WP_000516537.1	hypothetical protein	-
LMB17_RS26930 (LMB17_26940)	16092..16385	+	294	WP_001677496.1	hypothetical protein	-
LMB17_RS26935 (LMB17_26945)	16395..16766	+	372	WP_247150869.1	hypothetical protein	-
LMB17_RS26940 (LMB17_26950)	16748..17680	+	933	WP_000057449.1	hypothetical protein	-
LMB17_RS26945 (LMB17_26955)	17677..18039	+	363	WP_001261544.1	hypothetical protein	-
LMB17_RS26950 (LMB17_26960)	18114..18251	-	138	WP_000123562.1	hypothetical protein	-
LMB17_RS26955 (LMB17_26965)	18701..18952	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
LMB17_RS26960 (LMB17_26970)	19076..19465	+	390	WP_000506726.1	S24 family peptidase	-
LMB17_RS26965 (LMB17_26975)	19538..19759	+	222	WP_032271830.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LMB17_RS26970 (LMB17_26980)	19759..20139	+	381	WP_165467778.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LMB17_RS26975 (LMB17_26985)	20144..20323	+	180	WP_001339207.1	hypothetical protein	-
LMB17_RS26980 (LMB17_26990)	20351..21394	+	1044	WP_023356283.1	DUF968 domain-containing protein	-
LMB17_RS26985 (LMB17_26995)	21483..21935	+	453	WP_001326849.1	late promoter-activating protein	-
LMB17_RS26990 (LMB17_27000)	22021..23214	+	1194	WP_139496163.1	terminase	-
LMB17_RS26995 (LMB17_27005)	23214..24698	+	1485	WP_072665123.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97374	97374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13598.33 Da
• Isoelectric Point: 5.1514

>T222818 WP_165467778.1 NZ_CP086223:19759-20139 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGPAE

Nucleotide

• Length: 381 bp

>T222818 NZ_CP118143:439991-440272 [Pseudomonas chlororaphis]
ATGACTTATAAGCTCCAGTTTCTGCCTTCGGCACGTAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGTCCGCGGATGCCCTTCACGGCATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGACTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTGGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAACGCAGCGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8161.16 Da
• Isoelectric Point: 5.1106

>AT222818 WP_032271830.1 NZ_CP086223:19538-19759 [Escherichia coli]
MRSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT222818 NZ_CP118143:439756-440001 [Pseudomonas chlororaphis]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAACCCCTCGGCTGTCCTGAGCGGCGCACA
CGGTGGTCCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGGCACGAGACCCCAGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A765X875