Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 87764..89387 Replicon plasmid p779-1-MCR
Accession NZ_CP086221
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LMB17_RS25275 Protein ID WP_000088046.1
Coordinates 88062..89387 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LMB17_RS25270 Protein ID WP_024136327.1
Coordinates 87764..88057 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS25245 (LMB17_25250) 84032..85006 - 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
LMB17_RS25250 (LMB17_25255) 85440..85721 - 282 WP_000077926.1 hypothetical protein -
LMB17_RS25255 (LMB17_25260) 85771..85962 - 192 WP_000398480.1 hypothetical protein -
LMB17_RS25260 (LMB17_25265) 86054..86395 - 342 WP_001151575.1 hypothetical protein -
LMB17_RS25265 (LMB17_25270) 86768..87160 + 393 WP_000341066.1 hypothetical protein -
LMB17_RS25270 (LMB17_25275) 87764..88057 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LMB17_RS25275 (LMB17_25280) 88062..89387 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LMB17_RS25280 (LMB17_25285) 89448..89654 + 207 WP_000134171.1 hypothetical protein -
LMB17_RS25285 (LMB17_25290) 89756..90166 + 411 WP_000985911.1 hypothetical protein -
LMB17_RS25290 (LMB17_25295) 90179..90994 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
LMB17_RS25295 (LMB17_25300) 91248..91673 + 426 WP_001043843.1 hypothetical protein -
LMB17_RS25300 (LMB17_25305) 92127..92362 + 236 Protein_88 type I restriction-modification system subunit M N-terminal domain-containing protein -
LMB17_RS25305 (LMB17_25310) 92417..92716 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / sul1 - 1..242460 242460
- flank IS/Tn mcr-1.1 - 82211..84955 2744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T222814 WP_000088046.1 NZ_CP086221:88062-89387 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T222814 NZ_CP118142:4942699-4942989 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT222814 WP_024136327.1 NZ_CP086221:87764-88057 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT222814 NZ_CP118142:4942404-4942697 [Pseudomonas chlororaphis]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References