Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4345227..4345724 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LMB17_RS21055 Protein ID WP_001173343.1
Coordinates 4345551..4345724 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LMB17_RS21050 Protein ID WP_000398619.1
Coordinates 4345227..4345523 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS21030 (4341958) 4341958..4342677 - 720 WP_000611288.1 two-component system response regulator DcuR -
LMB17_RS21035 (4342674) 4342674..4344305 - 1632 WP_001216487.1 sensor histidine kinase -
LMB17_RS21040 (4344486) 4344486..4344716 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LMB17_RS21045 (4344728) 4344728..4345000 + 273 WP_000405649.1 GNAT family N-acetyltransferase -
LMB17_RS21050 (4345227) 4345227..4345523 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LMB17_RS21055 (4345551) 4345551..4345724 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LMB17_RS21060 (4345843) 4345843..4347360 - 1518 WP_001295074.1 lysine--tRNA ligase -
LMB17_RS21065 (4347597) 4347597..4349054 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T222811 WP_001173343.1 NZ_CP086220:4345551-4345724 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T222811 NZ_CP118141:c6266547-6266248 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCCCCGGTGGGCGAAGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT222811 WP_000398619.1 NZ_CP086220:4345227-4345523 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT222811 NZ_CP118141:c6266251-6265958 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAAACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGTGTGGTGGCGCGGGCCAAGGGCATGACTGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCATTCAGCGAAAAAGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGACTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References