Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3584668..3585299 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag LMB17_RS17565 Protein ID WP_001259388.1
Coordinates 3585024..3585299 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LMB17_RS17560 Protein ID WP_000593555.1
Coordinates 3584668..3585027 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS17535 (3580800) 3580800..3581744 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
LMB17_RS17540 (3581741) 3581741..3582574 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LMB17_RS17545 (3582574) 3582574..3583338 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LMB17_RS17550 (3583335) 3583335..3584141 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LMB17_RS17555 (3584147) 3584147..3584548 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LMB17_RS17560 (3584668) 3584668..3585027 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LMB17_RS17565 (3585024) 3585024..3585299 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
LMB17_RS17570 (3585372) 3585372..3586496 - 1125 WP_001314210.1 ABC transporter permease -
LMB17_RS17575 (3586496) 3586496..3589231 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
LMB17_RS17580 (3589228) 3589228..3590295 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T222809 WP_001259388.1 NZ_CP086220:c3585299-3585024 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T222809 NZ_CP118141:c2648303-2648010 [Pseudomonas chlororaphis]
ATGAATTACGAGATCCTGCAGACGACCGTCTTTGCCCGATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATATGGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACGCTGATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTACCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT222809 WP_000593555.1 NZ_CP086220:c3585027-3584668 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT222809 NZ_CP118141:c2648008-2647718 [Pseudomonas chlororaphis]
ATGTCCGACACACTGCTTGCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References