Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2753665..2754248 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag LMB17_RS13455 Protein ID WP_000254745.1
Coordinates 2753665..2754000 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LMB17_RS13460 Protein ID WP_000581937.1
Coordinates 2754000..2754248 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS13440 (2749552) 2749552..2750850 - 1299 WP_000036723.1 phosphopyruvate hydratase -
LMB17_RS13445 (2750938) 2750938..2752575 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LMB17_RS13450 (2752803) 2752803..2753594 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
LMB17_RS13455 (2753665) 2753665..2754000 - 336 WP_000254745.1 endoribonuclease MazF Toxin
LMB17_RS13460 (2754000) 2754000..2754248 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LMB17_RS13465 (2754326) 2754326..2756560 - 2235 WP_000226815.1 GTP pyrophosphokinase -
LMB17_RS13470 (2756608) 2756608..2757909 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T222804 WP_000254745.1 NZ_CP086220:c2754000-2753665 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T222804 NZ_CP118141:441590-441871 [Pseudomonas chlororaphis]
ATGACTTATAAGCTCCAGTTTCTACCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTACGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGATGCCCTTCACGGCATGGCGGACTGCTACAAGATCA
AGCTCAAAGCCTCTGGTTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGTAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT222804 WP_000581937.1 NZ_CP086220:c2754248-2754000 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT222804 NZ_CP118141:441355-441600 [Pseudomonas chlororaphis]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAGCAGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAATCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCGATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References