Detailed information of TA system TA222803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2459088..2459713
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMB17_RS12030
• Protein ID: WP_000911330.1
• Coordinates: 2459088..2459486 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LMB17_RS12035
• Protein ID: WP_000450524.1
• Coordinates: 2459486..2459713 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS12010 (2454995)	2454995..2455195	+	201	WP_000383836.1	YpfN family protein	-
LMB17_RS12015 (2455276)	2455276..2455974	-	699	WP_000679812.1	esterase	-
LMB17_RS12020 (2456048)	2456048..2458063	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
LMB17_RS12025 (2458078)	2458078..2458941	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
LMB17_RS12030 (2459088)	2459088..2459486	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMB17_RS12035 (2459486)	2459486..2459713	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LMB17_RS12040 (2459867)	2459867..2460580	-	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LMB17_RS12045 (2460793)	2460793..2461827	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
LMB17_RS12050 (2461844)	2461844..2462722	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LMB17_RS12055 (2462868)	2462868..2463440	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LMB17_RS12060 (2463440)	2463440..2463910	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T222803 WP_000911330.1 NZ_CP086220:c2459486-2459088 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T222803 NZ_CP118140:c6034092-6033694 [Pseudomonas chlororaphis]
ATGCTCAAGTACATGTTGGATACGAACATCTGTATTTTCACCATCAAGAACAAACCGGAGCGGGTTCGGGAGGCGTTCAA
CCGCCAGCACGGCCGGTTGTGCATCAGTTCGGTGACCTTGATGGAACTGATTTATGGAGCAGAAAAATCAGCGGCTCCTG
AGAGAAACCTGTCTGTCATAGAGGGCTTCGTGGCACGTCTGGAAGTGCTCGCGTATGACTACGAAGCTGCTATTCACAGC
GGTCAGTTGCGTGCCGAGTTGGCCAGGGCTGGTACGCCAATAGGTCCTTACGATCAGCTGATTGCCGGCCATGCCAGATC
CCAGGGATTGGTATTGGTGACCAACAATGTGCGCGAGTTTGAACGGGTGCCAGGGCTGCGTATTGAGGATTGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT222803 WP_000450524.1 NZ_CP086220:c2459713-2459486 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT222803 NZ_CP118140:c6034322-6034092 [Pseudomonas chlororaphis]
ATGGAACAAGGGTCGGTATTCAAAAGTAACCGCAGCCAGGCCATTCGAATGCCCAAGGCAGTGGCTCTCCCTGACGATGT
AACGCGGGTGGATATCGTTGCGGTAGGGCGTACCCGGATCATTACCCCGGCGGGGGAAGCCTGGGACAGCTGGTTCGATG
GCGAGTCGGTGAGCGCGGACTTCATGAATGAGCGGGATCAGCCTGCGGAACAAGAGCGTGAGGCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33