Detailed information of TA system TA222791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1231866..1232504
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LMB17_RS06055
• Protein ID: WP_000813794.1
• Coordinates: 1231866..1232042 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LMB17_RS06060
• Protein ID: WP_001270286.1
• Coordinates: 1232088..1232504 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS06035 (1227485)	1227485..1228699	-	1215	WP_001348055.1	BenE family transporter YdcO	-
LMB17_RS06040 (1228752)	1228752..1229288	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LMB17_RS06045 (1229361)	1229361..1231322	+	1962	WP_072644798.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LMB17_RS06050 (1231414)	1231414..1231644	-	231	WP_000494244.1	YncJ family protein	-
LMB17_RS06055 (1231866)	1231866..1232042	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LMB17_RS06060 (1232088)	1232088..1232504	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LMB17_RS06065 (1232583)	1232583..1233989	+	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
LMB17_RS06070 (1234234)	1234234..1235379	+	1146	WP_072644797.1	ABC transporter substrate-binding protein	-
LMB17_RS06075 (1235397)	1235397..1236410	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LMB17_RS06080 (1236411)	1236411..1237352	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1226297..1227445	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T222791 WP_000813794.1 NZ_CP086220:1231866-1232042 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T222791 NZ_CP118139:c2647754-2647572 [Pseudomonas chlororaphis]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAAAAGGATCTGCCACGGGGGACGGTCAGGGCCATCA
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT222791 WP_001270286.1 NZ_CP086220:1232088-1232504 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT222791 NZ_CP118139:c2647536-2647135 [Pseudomonas chlororaphis]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCGGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCATACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGACATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCATCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Toxin

• PDB: 6HPB