Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 208170..208788 Replicon chromosome
Accession NZ_CP086220
Organism Escherichia coli strain 779

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LMB17_RS00975 Protein ID WP_001291435.1
Coordinates 208170..208388 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LMB17_RS00980 Protein ID WP_000344800.1
Coordinates 208414..208788 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMB17_RS00940 (203460) 203460..204032 + 573 WP_000779826.1 YbaY family lipoprotein -
LMB17_RS00945 (204063) 204063..204374 - 312 WP_000409911.1 MGMT family protein -
LMB17_RS00955 (204753) 204753..205106 + 354 WP_000878141.1 DUF1428 family protein -
LMB17_RS00960 (205148) 205148..206698 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LMB17_RS00965 (206862) 206862..207332 - 471 WP_000136192.1 YlaC family protein -
LMB17_RS00970 (207448) 207448..207999 - 552 WP_000102564.1 maltose O-acetyltransferase -
LMB17_RS00975 (208170) 208170..208388 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LMB17_RS00980 (208414) 208414..208788 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LMB17_RS00985 (209334) 209334..212483 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LMB17_RS00990 (212506) 212506..213699 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222780 WP_001291435.1 NZ_CP086220:c208388-208170 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T222780 NZ_CP086220:c208388-208170 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT222780 WP_000344800.1 NZ_CP086220:c208788-208414 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT222780 NZ_CP086220:c208788-208414 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References