Detailed information of TA system TA222779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 173875..174712
• Replicon: chromosome
• Accession: NZ_CP086220
• Organism: Escherichia coli strain 779

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LMB17_RS00805
• Protein ID: WP_000227784.1
• Coordinates: 173875..174417 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LMB17_RS00810
• Protein ID: WP_001297137.1
• Coordinates: 174401..174712 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMB17_RS00785 (169423)	169423..170334	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
LMB17_RS00790 (170502)	170502..170993	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
LMB17_RS00795 (171121)	171121..172485	-	1365	WP_001000974.1	MFS transporter	-
LMB17_RS00800 (172893)	172893..173819	+	927	WP_077890888.1	tetratricopeptide repeat protein	-
LMB17_RS00805 (173875)	173875..174417	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LMB17_RS00810 (174401)	174401..174712	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LMB17_RS00815 (174897)	174897..175787	-	891	WP_000971336.1	heme o synthase	-
LMB17_RS00820 (175799)	175799..176128	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LMB17_RS00825 (176128)	176128..176742	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LMB17_RS00830 (176732)	176732..178723	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LMB17_RS00835 (178745)	178745..179692	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T222779 WP_000227784.1 NZ_CP086220:c174417-173875 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T222779 NZ_CP118137:c2627776-2627483 [Pseudomonas chlororaphis]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT222779 WP_001297137.1 NZ_CP086220:c174712-174401 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT222779 NZ_CP118137:c2627481-2627191 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3