Detailed information of TA system TA222778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2792036..2792565
• Replicon: chromosome
• Accession: NZ_CP086215
• Organism: Staphylococcus aureus strain Patient 14 Isolate 02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LLZ98_RS13895
• Protein ID: WP_000621175.1
• Coordinates: 2792203..2792565 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LLZ98_RS13890
• Protein ID: WP_000948331.1
• Coordinates: 2792036..2792206 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ98_RS13860 (2787073)	2787073..2787633	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
LLZ98_RS13865 (2787841)	2787841..2788320	+	480	WP_094659677.1	hypothetical protein	-
LLZ98_RS13870 (2788313)	2788313..2789896	+	1584	WP_001294626.1	PH domain-containing protein	-
LLZ98_RS13875 (2789883)	2789883..2790374	+	492	WP_001205912.1	PH domain-containing protein	-
LLZ98_RS13880 (2790378)	2790378..2790737	+	360	WP_000581197.1	holo-ACP synthase	-
LLZ98_RS13885 (2790803)	2790803..2791951	+	1149	WP_001281154.1	alanine racemase	-
LLZ98_RS13890 (2792036)	2792036..2792206	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LLZ98_RS13895 (2792203)	2792203..2792565	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LLZ98_RS13900 (2792915)	2792915..2793916	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
LLZ98_RS13905 (2794035)	2794035..2794361	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LLZ98_RS13910 (2794363)	2794363..2794842	+	480	WP_196506059.1	anti-sigma B factor RsbW	-
LLZ98_RS13915 (2794817)	2794817..2795587	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T222778 WP_000621175.1 NZ_CP086215:2792203-2792565 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T222778 NZ_CP118137:c2627481-2627191 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT222778 WP_000948331.1 NZ_CP086215:2792036-2792206 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT222778 NZ_CP118137:c2627776-2627483 [Pseudomonas chlororaphis]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1