Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5771431..5772045 Replicon chromosome
Accession NZ_CP086213
Organism Pseudomonas aeruginosa strain Pa3

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag LLJ53_RS27375 Protein ID WP_071534354.1
Coordinates 5771863..5772045 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag LLJ53_RS27370 Protein ID WP_012077229.1
Coordinates 5771431..5771835 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLJ53_RS27345 (LLJ53_27340) 5767571..5768281 + 711 WP_012074129.1 hypothetical protein -
LLJ53_RS27350 (LLJ53_27345) 5768278..5769198 + 921 WP_012074128.1 hypothetical protein -
LLJ53_RS27355 (LLJ53_27350) 5769195..5769734 + 540 WP_012074127.1 hypothetical protein -
LLJ53_RS27360 (LLJ53_27355) 5769734..5770432 + 699 WP_033896049.1 hypothetical protein -
LLJ53_RS27365 (LLJ53_27360) 5770417..5771388 + 972 WP_012077228.1 hypothetical protein -
LLJ53_RS27370 (LLJ53_27365) 5771431..5771835 - 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LLJ53_RS27375 (LLJ53_27370) 5771863..5772045 - 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
LLJ53_RS27380 (LLJ53_27375) 5772588..5773520 - 933 WP_003120918.1 ZIP family metal transporter -
LLJ53_RS27385 (LLJ53_27380) 5773539..5774150 - 612 WP_003098862.1 superoxide dismutase -
LLJ53_RS27390 (LLJ53_27385) 5774163..5774612 - 450 WP_003094369.1 hypothetical protein -
LLJ53_RS27395 (LLJ53_27390) 5774640..5776016 - 1377 WP_003098863.1 class II fumarate hydratase FumC -
LLJ53_RS27400 (LLJ53_27395) 5776009..5776404 - 396 WP_003162782.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5734551..5772045 37494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T222757 WP_071534354.1 NZ_CP086213:c5772045-5771863 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T222757 NZ_CP118135:c1981558-1981376 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT222757 WP_012077229.1 NZ_CP086213:c5771835-5771431 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT222757 NZ_CP118135:c1981346-1980945 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTCCTTGCCGCAGCGGCATTGCGCGAGTTATCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References