Detailed information of TA system TA222752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2069701..2071209
• Replicon: chromosome
• Accession: NZ_CP086213
• Organism: Pseudomonas aeruginosa strain Pa3

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: LLJ53_RS09815
• Protein ID: WP_003119682.1
• Coordinates: 2069701..2070288 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A6V2F0
• Locus tag: LLJ53_RS09820
• Protein ID: WP_012074952.1
• Coordinates: 2070355..2071209 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLJ53_RS09810 (LLJ53_09820)	2065956..2069435	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
LLJ53_RS09815 (LLJ53_09825)	2069701..2070288	+	588	WP_003119682.1	GNAT family protein	Toxin
LLJ53_RS09820 (LLJ53_09830)	2070355..2071209	+	855	WP_012074952.1	AraC family transcriptional regulator	Antitoxin
LLJ53_RS09825 (LLJ53_09835)	2071305..2073470	+	2166	WP_025297505.1	TonB-dependent receptor family protein	-
LLJ53_RS09830 (LLJ53_09840)	2073645..2075519	+	1875	WP_003117607.1	MFS transporter	-
LLJ53_RS09835 (LLJ53_09845)	2075580..2075789	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• DNA binding HTH domain, AraC-type (196-273)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T222752 WP_003119682.1 NZ_CP086213:2069701-2070288 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T222752 NZ_CP118134:4144495-4145067 [Pseudomonas synxantha]
ATGGACCTGGAAAAAATCAAAGGCCGTCTCGATTTCCTGCGCGAAGCGGAAAAACTCAAGGATGTGCTCAGAAGTGCCCA
CACGGCTTCGGGTCGCACTGAAAGCACGGCCGAACACAGTTGGCGCCTGTGTCTGATGGCGATGGTGTTCGCCGATCAAC
TGCCCGGCCTGGACCTGCTGAAAATCCTCAAGATGTGCGTTATCCATGATTTGGGCGAAGCCATCCACGGCGATATCCCG
GCGGTCAGCCAGGCGGCCTTTCCCAACAAGGGCGAACAGGAGCGACTCGACCTGCTGCTGTTGGCCCAGTCCCTGGACAG
CCCGCTGAAAACCGAAATCCTGACGTTGTGGGATGATTACGAGAACGCGCTTTCGCCGGAAGCAAAAGCGGTCAAGGCCA
TGGACAAACTGGAGACCCTGCTGCAGCACACCCAGGGGCTGAACCCTCCCGATTTCGACTACGCCTTCAACCTGGCCTAC
GGCAAAAAACACACGGATGCCGACCCCTTGTTCGAAGCATTGCGGGCATTGATCGATCGAGCGACAGAGCAAAGGCTTCA
CGCCGCCGAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31788.11 Da
• Isoelectric Point: 7.7066

>AT222752 WP_012074952.1 NZ_CP086213:2070355-2071209 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSGFALVFIEQGAQRFRHRGGEHLAPWGSMVLINPDEVHTGSK
AHDSGWLYRGYYPEVEQIASILDELEIRHGGVPRFADSVVHDQPLARAFVHLHRLLADHAGSLETQTCWRETLLALFRRH
ARLPEAPKAGSEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKNGRA
PLDVALELGFADQSHLGRQFKQAYGVAPGEYRAACARSSLTMCE

Nucleotide

• Length: 855 bp

>AT222752 NZ_CP118134:4143850-4144407 [Pseudomonas synxantha]
ATGCCTCACTCCTTGCTCAAGCCTGCGCTGCCCGGCATTGCGCTGCGGCGATGGCGTTTGCTGCACCGCGTCAAGCAAAG
CCATGCGGCGCAGCTATTCAACGTCACGCAATCGACTATTTCGCGCTGGGAAAGTGGCATACAAGCAATGGATCCGGCTG
CGCACCAACAGCTTGAGCAACTGTTGGCAGCCCGGCTTGATGCAGCCGCCGACCAGGCATTGGCCCGGCTGGTCAGTGAG
AGTTCGCAAGCGATGCACCTGGTATGCGACCTGACGCACCGCCTGCTCGCATGCTCGCCCTCCCGCGCACTCCAATTCAC
CAGCCCGCTCACCGACCTTATGGGCCAGTCGCTGTGGCGCTTCGCAACCCAGGAAATCCAGCTGATCGAAGCCAGCCTGG
AGCACACCGGCTGGCGCGAAACCCAGGCACCCCCAGCCCTTGAGTTCACCACTGGAAGCAACGATTCCAGCCTCGTGCCG
ATACGCCCAAGCCTGTGTCGCTGGACGCGCATGACCCTGAGCGATGGCAGCGCCGCGCGCCTGGTCGAGACGCTCTGA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ

Antitoxin

• AlphaFold DB: A6V2F0