Detailed information of TA system TA222751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 160172..160677
• Replicon: chromosome
• Accession: NZ_CP086213
• Organism: Pseudomonas aeruginosa strain Pa3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LLJ53_RS00780
• Protein ID: WP_003083773.1
• Coordinates: 160172..160453 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LLJ53_RS00785
• Protein ID: WP_003083775.1
• Coordinates: 160450..160677 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLJ53_RS00755 (LLJ53_00760)	155423..156772	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
LLJ53_RS00760 (LLJ53_00765)	156821..157507	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LLJ53_RS00765 (LLJ53_00770)	157608..158342	+	735	WP_004346926.1	GntR family transcriptional regulator	-
LLJ53_RS00770 (LLJ53_00775)	158522..158932	+	411	WP_003101225.1	aegerolysin family protein	-
LLJ53_RS00775 (LLJ53_00780)	158964..159872	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LLJ53_RS00780 (LLJ53_00785)	160172..160453	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LLJ53_RS00785 (LLJ53_00790)	160450..160677	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LLJ53_RS00790 (LLJ53_00795)	160853..161473	-	621	WP_003101226.1	hypothetical protein	-
LLJ53_RS00795 (LLJ53_00800)	161574..162074	+	501	WP_003101228.1	LEA type 2 family protein	-
LLJ53_RS00800 (LLJ53_00805)	162147..162488	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LLJ53_RS00805 (LLJ53_00810)	162570..163997	-	1428	WP_003083784.1	GABA permease	-
LLJ53_RS00810 (LLJ53_00815)	164166..165659	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T222751 WP_003083773.1 NZ_CP086213:c160453-160172 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T222751 NZ_CP118134:3898328-3898678 [Pseudomonas synxantha]
ATGGATGCTCTATTTATCGAACTGCCGGCATTCGAGCGGCATCGCAAGGACTATCTGAGTGAGGAGCTTTTCCAGGGTTT
TCAACAGGAGTTAATGAAAAACCCCGAAGCAGGAGATGTGATCGAAGGAACGGGCGGGTTGAGAAAGGTTCGCTTCGTTG
ATGAACGGCGAAACAAAGGCAAGCGTGGTGGCCTGCGAGTCATTTACTACTGGTGGTCAGGCGGCACGCAATTCTGGTTA
TTCACCTTGTACGGCAAGCACGAACATAGCGACCTGACACCCCACCACAAGAAAGCTCTTAAACACATGCTGGACAGGGA
AATCAAAGCGAGAACACATCATGAAACGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT222751 WP_003083775.1 NZ_CP086213:c160677-160450 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT222751 NZ_CP118134:3898668-3898982 [Pseudomonas synxantha]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGCTTCGACGCCCTGGCCGATGAGCGCCAGGGTAAAATCACCCTGCG
CAACCACAAGGTCCACCTCAACAAGCTGGCGCCGCTCACCGCTGATGAGGTGATCGCCGTGCGCCAGCAACTCAACCTTT
CCCGGTCGGTATTCGCCATGTACCTGCGTACCAACACCCGCACCCTGGAGAACTGGGAACAAGGCCGGGCCAAGCCCAAC
GCCCAGGCCGTCACCCTGATCCGTCTGGTAGCGCGTTTTCCTGAAACCGTGGCGCAACTTGCCGCATTGGGCTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9