Detailed information of TA system TA222750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200706..201342
• Replicon: chromosome
• Accession: NZ_CP086208
• Organism: Priestia sp. J2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: LJY09_RS01130
• Protein ID: WP_013055004.1
• Coordinates: 200992..201342 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: LJY09_RS01125
• Protein ID: WP_013055003.1
• Coordinates: 200706..200987 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY09_RS01100	196072..197043	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
LJY09_RS01105	197052..197654	-	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
LJY09_RS01110	197719..198084	+	366	WP_028411881.1	holo-ACP synthase	-
LJY09_RS01115	198143..199201	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
LJY09_RS01120	199315..200505	+	1191	WP_034327253.1	alanine racemase	-
LJY09_RS01125	200706..200987	+	282	WP_013055003.1	hypothetical protein	Antitoxin
LJY09_RS01130	200992..201342	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LJY09_RS01135	201500..202336	+	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
LJY09_RS01140	202339..202695	+	357	WP_013055006.1	STAS domain-containing protein	-
LJY09_RS01145	202699..203100	+	402	WP_013055007.1	anti-sigma regulatory factor	-
LJY09_RS01150	203132..204124	+	993	WP_223260212.1	PP2C family protein-serine/threonine phosphatase	-
LJY09_RS01155	204184..204516	+	333	WP_013055009.1	anti-sigma factor antagonist	-
LJY09_RS01160	204513..204998	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
LJY09_RS01165	204964..205758	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T222750 WP_013055004.1 NZ_CP086208:200992-201342 [Priestia sp. J2]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T222750 NZ_CP118134:c2687018-2686767 [Pseudomonas synxantha]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTTACCTGGCCCGAGCTCGTCACACTGTT
ACGACGGCTCGGATACACACAGATTGAAGGGGCCGGAAGCCGGGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CGTTGCACAGGCCTCACCCCGGCAACGAACTGAAGCACTACATTCGTCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT222750 WP_013055003.1 NZ_CP086208:200706-200987 [Priestia sp. J2]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT222750 NZ_CP118134:c2686770-2686405 [Pseudomonas synxantha]
GTGAACAACCAACTGAAACACAATGGCTATATCGGCTCTATCGAAGCCAGCCTTGAAGACAACTGCCTGTTTGGCAAGGT
CCTGTTCATCAAGGCATTAGTGAGCTTCGAAGGTAAAACCGTCGCAGAACTGGAAGCCGCCTTCCGTGAAGCGGTGGACG
ATTACCTCGCGACCTGCCAGGCCCTCGGGCAAACGCCGGAGAAGCCTTGCAAAGGTTCATTCAACGTGCGCGTCGGGCAT
GACCTGCACTTGGCAGCAGCCCTGGCAGCAACCCGTAAGAAAGTGACGTTGAATGATCTGACGCGCCAAGCGTTGAGCGA
GTTTCTACAACATCACTATCCAGAGCCATGCCCGGCGATAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9