Detailed information of TA system TA222748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2466371..2466978
• Replicon: chromosome
• Accession: NZ_CP086206
• Organism: Pseudomonas sp. HN8-3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5D3G4M7
• Locus tag: LJX92_RS11010
• Protein ID: WP_032876316.1
• Coordinates: 2466727..2466978 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LJX92_RS11005
• Protein ID: WP_103735389.1
• Coordinates: 2466371..2466730 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX92_RS10985 (LJX92_11000)	2461896..2463251	+	1356	WP_078821098.1	MATE family efflux transporter	-
LJX92_RS10990 (LJX92_11005)	2463235..2463822	-	588	WP_032876325.1	TetR/AcrR family transcriptional regulator	-
LJX92_RS10995 (LJX92_11010)	2463954..2465126	+	1173	WP_228309750.1	MFS transporter	-
LJX92_RS11000 (LJX92_11015)	2465139..2466143	-	1005	WP_103735388.1	family 2 encapsulin nanocompartment cargo protein terpene cyclase	-
LJX92_RS11005 (LJX92_11020)	2466371..2466730	-	360	WP_103735389.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LJX92_RS11010 (LJX92_11025)	2466727..2466978	-	252	WP_032876316.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9503.14 Da
• Isoelectric Point: 10.0459

>T222748 WP_032876316.1 NZ_CP086206:c2466978-2466727 [Pseudomonas sp. HN8-3]
VSKNEKLLAKLLNEHMAFTWPELVTLLRRLGYTLIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T222748 NZ_CP118108:1330576-1330926 [Paenibacillus urinalis]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCGGATCTTTCACCCGTTGTCGGTTCCGAGCAAGGGGGAGTCAGACCGGT
GCTTGTGATCCAGAATGATATTGGCAACCGTTTCAGTCCTACTTGTATCGTTGCGGCCATCACGGCTCAGATTCAGAAGG
CGAAGCTGCCGACCCACGTGGAAATTGATGCAGAACTGCACGGTTTTGATCGGGATTCTGTTGTGTTGCTCGAACAGATT
AGAACGATTGACAAACAGCGTCTGACTGACAAGATTACGCATTTAGATGATGATACGATGAAGCTTGTGGGTGAAGCTCT
ACAGATCAGTCTGGGATTAATTGATTTCTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13016.86 Da
• Isoelectric Point: 6.4733

>AT222748 WP_103735389.1 NZ_CP086206:c2466730-2466371 [Pseudomonas sp. HN8-3]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCQTLGQSPEKPCKGSFNVRVGH
ELHLAAALAAMRKKVTLNDLTRQALNEFLQQHDDGLAAV

Nucleotide

• Length: 360 bp

>AT222748 NZ_CP118108:1330291-1330572 [Paenibacillus urinalis]
TTGACCAATGTACAGAACACCAAACGGATTATGATTAGTTTGCCGGATCATCTCTTACAGGAAGTAGATGGTATTGTAGC
AATGGAGAATTCGAACCGCAGTGAATTCATCAGGCAAGCCATGAAGCTGTACTTAAACGAACGGAAGAAGCGTTATATCC
GGGAGTCAATGCAGCGAGGCTACATGGAAATGGCCAAGATTAATTTGACCATGGCTTCTGAAGCTTTTCACGCGGAGGAT
GATGCAGACAGCACTCTGGGCCGCTTAGTGAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A5D3G4M7