Detailed information of TA system TA222746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1652185..1652707
• Replicon: chromosome
• Accession: NZ_CP086206
• Organism: Pseudomonas sp. HN8-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LJX92_RS07465
• Protein ID: WP_103733512.1
• Coordinates: 1652185..1652478 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0W0N4Z1
• Locus tag: LJX92_RS07470
• Protein ID: WP_014717442.1
• Coordinates: 1652468..1652707 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX92_RS07435 (LJX92_07445)	1647578..1648138	-	561	WP_003189463.1	glycine cleavage system protein R	-
LJX92_RS07440 (LJX92_07450)	1648401..1649279	+	879	WP_032877264.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LJX92_RS07445 (LJX92_07455)	1649297..1650412	+	1116	WP_005786026.1	outer membrane protein assembly factor BamC	-
LJX92_RS07450 (LJX92_07460)	1650417..1651175	+	759	WP_228309512.1	MBL fold metallo-hydrolase	-
LJX92_RS07455 (LJX92_07465)	1651203..1651916	+	714	WP_005786030.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LJX92_RS07465 (LJX92_07475)	1652185..1652478	-	294	WP_103733512.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJX92_RS07470 (LJX92_07480)	1652468..1652707	-	240	WP_014717442.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LJX92_RS07475 (LJX92_07485)	1653359..1653613	+	255	WP_032877263.1	hypothetical protein	-
LJX92_RS07480 (LJX92_07490)	1653687..1654913	-	1227	WP_032877262.1	MFS transporter	-
LJX92_RS07485 (LJX92_07495)	1655153..1655440	-	288	WP_003189536.1	NadS family protein	-
LJX92_RS07490 (LJX92_07500)	1655443..1655757	-	315	WP_057022036.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LJX92_RS07495 (LJX92_07505)	1655863..1656804	-	942	WP_216090883.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11684.52 Da
• Isoelectric Point: 10.2570

>T222746 WP_103733512.1 NZ_CP086206:c1652478-1652185 [Pseudomonas sp. HN8-3]
MTYSLDFDARALKEWQKLGDTLRQQLKKKLMEILNNPRIEANRLHGLPDCYKIKLRSSRYRLVYQVIDQEVTVFVVAIDK
RERDQAYRKASERLDQR

Nucleotide

• Length: 294 bp

>T222746 NZ_CP118101:1458347-1458697 [Paenibacillus urinalis]
TTGATCGTTAAACGCGGCGACGTTTTTTTTGCGGATCTTTCACCCGTTGTCGGTTCCGAGCAAGGGGGAGTCAGACCGGT
GCTTGTGATCCAGAATGATATTGGCAACCGTTTCAGTCCTACTTGTATCGTTGCGGCCATCACGGCTCAGATTCAGAAGG
CGAAGCTGCCGACCCACGTGGAAATTGATGCAGAACTGCACGGTTTTGATCGGGATTCTGTTGTGTTGCTCGAGCAGATT
AGAACGATTGACAAACAGCGTCTGACTGACAAGATTACGCATTTAGATGATGATACGATGAAGCTTGTGGGTGAAGCTCT
ACAGATCAGTCTGGGATTAATTGATTTCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8997.40 Da
• Isoelectric Point: 4.6017

>AT222746 WP_014717442.1 NZ_CP086206:c1652707-1652468 [Pseudomonas sp. HN8-3]
MASINIRIDDDLKARAYLELEKLGVTPSELIRQALQYVAERGQLPFKPVLMTEEDEALMATVRERLASPQRVRVSLDDL

Nucleotide

• Length: 240 bp

>AT222746 NZ_CP118101:1458062-1458343 [Paenibacillus urinalis]
TTGACCAATGTACAGAACACCAAACGGATTATGATTAGTTTGCCGGATCATCTCTTACAGGAAGTAGATGGTATTGTAGC
AATGGAGAATTCGAACCGCAGTGAATTCATCAGGCAAGCCATGAAGCTGTACTTAAACGAACGGAAGAAGCGTTATATCC
GGGAGTCGATGCAGCGAGGCTATATGGAAATGGCCAAGATTAATTTGACCATGGCTTCTGAAGCTTTTCACGCGGAGGAT
GATGCAGACAGCACTCTGGGCCGCTTAGTGAGCGGGGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0W0N4Z1