Detailed information of TA system TA222745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1609422..1610037
• Replicon: chromosome
• Accession: NZ_CP086206
• Organism: Pseudomonas sp. HN8-3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LJX92_RS07245
• Protein ID: WP_078820528.1
• Coordinates: 1609855..1610037 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A5D3GIE6
• Locus tag: LJX92_RS07240
• Protein ID: WP_032877288.1
• Coordinates: 1609422..1609832 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX92_RS07205 (LJX92_07215)	1604972..1605448	-	477	WP_032877293.1	DMT family transporter	-
LJX92_RS07210 (LJX92_07220)	1605526..1606443	+	918	WP_228309505.1	LysR substrate-binding domain-containing protein	-
LJX92_RS07215 (LJX92_07225)	1606459..1606875	+	417	WP_005785954.1	fosfomycin resistance glutathione transferase	-
LJX92_RS07220 (LJX92_07230)	1606923..1607564	+	642	WP_032877291.1	LysE family translocator	-
LJX92_RS07225 (LJX92_07235)	1607683..1608210	+	528	WP_005785958.1	DUF4142 domain-containing protein	-
LJX92_RS07230 (LJX92_07240)	1608264..1608680	-	417	WP_032877290.1	low affinity iron permease family protein	-
LJX92_RS07235 (LJX92_07245)	1608782..1609282	-	501	WP_223018525.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
LJX92_RS07240 (LJX92_07250)	1609422..1609832	-	411	WP_032877288.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LJX92_RS07245 (LJX92_07255)	1609855..1610037	-	183	WP_078820528.1	type II toxin-antitoxin system HicA family toxin	Toxin
LJX92_RS07250 (LJX92_07260)	1610205..1611098	-	894	WP_103733495.1	LysR family transcriptional regulator	-
LJX92_RS07255 (LJX92_07265)	1611194..1612363	+	1170	WP_223018526.1	MFS transporter	-
LJX92_RS07265 (LJX92_07275)	1612643..1613527	-	885	WP_078820531.1	alpha/beta hydrolase	-
LJX92_RS07270 (LJX92_07280)	1613830..1614522	-	693	WP_228309506.1	pseudouridine synthase	-
LJX92_RS07275 (LJX92_07285)	1614522..1614734	-	213	WP_103733497.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6840.04 Da
• Isoelectric Point: 10.9586

>T222745 WP_078820528.1 NZ_CP086206:c1610037-1609855 [Pseudomonas sp. HN8-3]
VDSRYLIGNIVADGWYLVRIRGSHHHFKHRTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T222745 NZ_CP118097:c2169640-2168873 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAAACTAGAAGAATTTAGTCAAGATAATTTTGAACAAGCCTCCAAACGACTCGTTCGTTCTTTAACTCGTGGAAAAAC
AACCTCATCTCAGCCTAAGGCTATTTTGCTCGGTGGACAGAGTGGTGCTGGGAAAACAACGATCCATCGAATTAAACAAA
GGGAATTTCAAGGTAATATCATTATTATTGATGGAGATAGTTATCGCTCTTTTCATCCGAACTACCTTGGCCTACAGGAA
AGGTATGGCAAAGATAGTGTGGATTATACCAAAGTATTCGCGGGACAAATGGTTGAATATCTTGTAGATGAGTTGAGTAA
AAAAGGTTATCATTTATTAATCGAGGGTACCTTGAGAACAACAGAAGTTCCCAAAAAAACTGCACAATTATTGACAACTA
AAGGTTACCAAGTCTCACTTGCAGTAATTGCGACCAAGCCAGAATTGTCCTATCTCAGTACCTTGATACGTTATGAAGAG
CTCCATGCTATAGACCCTAGTCAAGCTAGAGCGACTCCCAAAGAGCACCATGATGGAATTGTAGAACATTTAGTTGATAA
CTTACGAGAGTTAGAAAATGACAAAATTTTTAATCAAATTCAAATTTATCAAAGAGATCAATCGTGTATTTATGATTCGC
AAGTAGATCAAATCTCAGCAGCAGAAGTTTTACAAGAATGCCTCTTCGGAAAATGGAGTAAGGTGGAGGAAGAGATGTTG
AAGTTGGGACAGGAACGGTTGAAAGAGCTGGGGGAAATTGATAAGTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14769.64 Da
• Isoelectric Point: 4.2941

>AT222745 WP_032877288.1 NZ_CP086206:c1609832-1609422 [Pseudomonas sp. HN8-3]
MLYPIAISTGDEDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDDYVLNHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT222745 NZ_CP118097:c2170116-2169640 [Streptococcus dysgalactiae subsp. equisimilis]
ATGATTGGAGAAAATATTAAAACGTTACGCAAGGCACATAATCTAACTCAGCCTGAGTTCGCAAAGATTGTTGGTATCTC
TCGAAATAGTCTGAGTCGTTATGAAAATGGGACAAGTCGAGTTTCGACAGAATTAATAGATCGTATCTGCAAGAAATTTA
ATGTTTCTTATATCGATATCGTAGGGGAGGAAAAAATGCTCACACCAGTAGAAGATTATCAGTTAACTCTGAAAATTGAA
GTGATTAAAGAACGAGGGGCGAATATCTTAGCGCAACTTTATCGTTTTCAAGATGAACAAGGAATTGCATTTGATGATAC
TTCTAATCCTTGGGTTCTAATGAGCGATGATTTGTCTGATTTAATTAACACGAAGATTTATCTTGTTGCAACTTTTGAGG
ATATAGAGCGATTTAATGGCTATTTGGATGGCATTGAACGTATGCTAGAACAAGCTACTCATCTGGTGGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D3GIE6