Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22971..23614 Replicon plasmid pF1057
Accession NZ_CP086202
Organism Enterobacter roggenkampii strain F1057

Toxin (Protein)


Gene name vagD Uniprot ID A0A3A3ZBE4
Locus tag LL005_RS22315 Protein ID WP_007374381.1
Coordinates 22971..23387 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID G8LQB1
Locus tag LL005_RS22320 Protein ID WP_007853351.1
Coordinates 23384..23614 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL005_RS22290 (LL005_22335) 18455..18793 - 339 WP_007374386.1 carboxymuconolactone decarboxylase family protein -
LL005_RS22295 (LL005_22340) 18810..19379 - 570 WP_042936984.1 TetR/AcrR family transcriptional regulator -
LL005_RS22300 (LL005_22345) 19563..20120 - 558 WP_007374384.1 OsmC family protein -
LL005_RS22305 (LL005_22350) 20329..21351 - 1023 WP_233396712.1 DNA helicase UvrD -
LL005_RS22310 (LL005_22355) 21336..22898 - 1563 WP_233396713.1 AAA family ATPase -
LL005_RS22315 (LL005_22360) 22971..23387 - 417 WP_007374381.1 type II toxin-antitoxin system VapC family toxin Toxin
LL005_RS22320 (LL005_22365) 23384..23614 - 231 WP_007853351.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LL005_RS22325 (LL005_22370) 24160..24462 + 303 WP_209702748.1 hypothetical protein -
LL005_RS22330 (LL005_22375) 24532..25350 + 819 WP_233396714.1 hypothetical protein -
LL005_RS22335 (LL005_22380) 25350..26126 + 777 WP_233396715.1 site-specific integrase -
LL005_RS22340 (LL005_22385) 26312..26617 - 306 WP_156566079.1 hypothetical protein -
LL005_RS22345 (LL005_22390) 26614..27240 - 627 WP_045895775.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / qacE / sul1 - 1..125002 125002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15105.57 Da        Isoelectric Point: 7.8882

>T222740 WP_007374381.1 NZ_CP086202:c23387-22971 [Enterobacter roggenkampii]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYAEMRFGATGPKAAPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T222740 NZ_CP118092:692979-693242 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTTAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAAAAATTAGTACCGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATTACGAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8674.77 Da        Isoelectric Point: 4.4692

>AT222740 WP_007853351.1 NZ_CP086202:c23614-23384 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT222740 NZ_CP118092:692728-692982 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAAGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A3ZBE4

Antitoxin

Source ID Structure
AlphaFold DB A0A3A3Z894

References