Detailed information of TA system TA222716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 439845..440336
• Replicon: chromosome
• Accession: NZ_CP086198
• Organism: Macrococcus canis strain KW0150

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LL975_RS02185
• Protein ID: WP_086043355.1
• Coordinates: 439986..440336 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LL975_RS02180
• Protein ID: WP_101495293.1
• Coordinates: 439845..439985 (+)
• Length: 47 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL975_RS02155 (LL975_02145)	435835..436311	+	477	WP_254254876.1	PH domain-containing protein	-
LL975_RS02160 (LL975_02150)	436301..437812	+	1512	WP_236365978.1	PH domain-containing protein	-
LL975_RS02165 (LL975_02155)	437796..438317	+	522	WP_236365977.1	PH domain-containing protein	-
LL975_RS02170 (LL975_02160)	438259..438615	+	357	WP_086043357.1	holo-ACP synthase	-
LL975_RS02175 (LL975_02165)	438641..439783	+	1143	WP_086043356.1	alanine racemase	-
LL975_RS02180 (LL975_02170)	439845..439985	+	141	WP_101495293.1	antitoxin MazE	Antitoxin
LL975_RS02185 (LL975_02175)	439986..440336	+	351	WP_086043355.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LL975_RS02190 (LL975_02180)	440385..441392	+	1008	WP_086043354.1	PP2C family protein-serine/threonine phosphatase	-
LL975_RS02195 (LL975_02185)	441473..441796	+	324	WP_086043353.1	anti-sigma factor antagonist	-
LL975_RS02200 (LL975_02190)	441801..442244	+	444	WP_086043352.1	anti-sigma B factor RsbW	-
LL975_RS02205 (LL975_02195)	442249..443016	+	768	WP_133420462.1	RNA polymerase sigma factor SigB	-
LL975_RS02210 (LL975_02200)	443093..445228	+	2136	WP_254254875.1	Tex family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12705.88 Da
• Isoelectric Point: 9.9423

>T222716 WP_086043355.1 NZ_CP086198:439986-440336 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Nucleotide

• Length: 351 bp

>T222716 NZ_CP118048:2310061-2310720 [Enterococcus faecalis]
TTGATTGGACTGTTGTGGGTGGATTTTATTAGTAAAAAACTCTATTTTGAAGCATTTAATTTTTCAAACAAATTAATAAA
AGAAGTATCTTACTATTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
ATGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATCTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAAGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAGGTTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACCCATACTTTTTCTGATATTCTTTTGAAAAATGGTTACTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGCATGTTAATGGCAAATGACCAAGCATTGTTTTACGCATTAAAAAAGTTT
TATTCATTTGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATCGAGCATCTTATATA
TGTAAATAACTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTTAATGATTCATAA

Antitoxin

Protein

• Length: 47 a.a.
• Molecular weight: 5319.89 Da
• Isoelectric Point: 3.8301

>AT222716 WP_101495293.1 NZ_CP086198:439845-439985 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE

Nucleotide

• Length: 141 bp

>AT222716 NZ_CP118048:2309681-2310064 [Enterococcus faecalis]
GTGCAACCATTAGCAAAAAGACTACAGCTTTTAAGAGAAGAAAAAGAATGGACAAAAACATATGTTGCAAAACAACTAGG
TTTAAATAACTTAGGAACCTATGCAAACTGGGAATATGGAACACGTGAACCAGATTCGGAAATGCTATCAAAAATTGCTT
CCTTATATGATGTATCTACCGATTTTTTACTAGGTAGAACAGAGGAAAGAATACATCTAAACGACAAGGAAAAAATTGGA
AAAAATATCATTTCTCATTTTCGTTTAAATACTTCTGATATGGACATAGAAGATATAGAAGAATTAGAAGAAGAATTAAT
CGATTTTCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGAAACGTAACAAAAAAGATTGA