Detailed information of TA system TA222713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3253865..3254610
• Replicon: chromosome
• Accession: NZ_CP086197
• Organism: Xanthomonas oryzae strain JS97-2

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LL929_RS15160
• Protein ID: WP_199285672.1
• Coordinates: 3253865..3254194 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LL929_RS15165
• Protein ID: WP_011407754.1
• Coordinates: 3254191..3254610 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL929_RS15130 (LL929_15115)	3248996..3249667	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
LL929_RS15140 (LL929_15125)	3249969..3250760	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
LL929_RS15145 (LL929_15130)	3250806..3251732	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
LL929_RS15150 (LL929_15135)	3252064..3252399	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LL929_RS15155 (LL929_15140)	3252443..3253762	-	1320	WP_011407756.1	IS701 family transposase	-
LL929_RS15160 (LL929_15145)	3253865..3254194	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
LL929_RS15165 (LL929_15150)	3254191..3254610	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
LL929_RS15170 (LL929_15155)	3254662..3255629	+	968	WP_011258188.1	IS5-like element ISXo1 family transposase	-
LL929_RS15175 (LL929_15160)	3255782..3256420	+	639	WP_011407753.1	phosphate phosphatase	-
LL929_RS15180 (LL929_15165)	3256458..3257693	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
LL929_RS15185 (LL929_15170)	3257995..3258321	+	327	Protein_2904	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3252443..3257693	5250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T222713 WP_199285672.1 NZ_CP086197:3253865-3254194 [Xanthomonas oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T222713 NZ_CP118046:c1537695-1537330 [Streptococcus anginosus]
GTGCATAATATCTATTTTTACAAGGATAAAAACGGTAATGAGCCTATTTTAGATTATATGCGAGAGCTTGCCAGTAAAAA
GGGAAAAGATAGTAGAATTAAACTCAATAAAATCAATGATTATATTGAGTTGTTAAGTCAACACGGAACACGCACAGGCG
AGCCATATATTAAGCATTTAGATGCTGAAATTTGGGAACTAAGACCATTAAGGGATAGAATTTTATTTGTTGCATGGATT
GATGGTAGCTTTGTTTTACTCCATCATTTCATGAAAAAGACACAGAAAACACCAAAAAGAGAGATTGAACAAGCTAAACG
TGAGTTAGCAGACTTAAAAGAAAGAGGTTTAGACAATGAAAAATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT222713 WP_011407754.1 NZ_CP086197:3254191-3254610 [Xanthomonas oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT222713 NZ_CP118046:c1537340-1537047 [Streptococcus anginosus]
ATGAAAAATAGTGCAATTGGTAGTAACTGGAAAGATGTAAGAGCTGAGTTATTCAGCAAAGAAGAAATTTTAGAAAGTGA
TATGCGCGTGGCTATCATGAGTGAGCTTATCGAGGCTAGGAATGAAAGGGGCATTAGTCAAAAAAAACTAGAGGAGCTGA
GTGGCGTTAGTCAGCCAGTCATAGCTAGAATGGAGACAGGAAAAACAAGCCCACAGCTTGATACAGTATTAAAGGTTTTG
GCAAGTCTTGATAAGACTTTAGCGGTTGTACCACTAGAGCGTGAACAAGTCTAG