Detailed information of TA system TA022271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2192598..2193127
• Replicon: chromosome
• Accession: NC_009641
• Organism: Staphylococcus aureus subsp. aureus str. Newman

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: NWMN_RS11420
• Protein ID: WP_000621175.1
• Coordinates: 2192598..2192960 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: NWMN_RS11425
• Protein ID: WP_000948331.1
• Coordinates: 2192957..2193127 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWMN_RS11395 (NWMN_1970)	2189575..2190345	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
NWMN_RS11400 (NWMN_1971)	2190320..2190799	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
NWMN_RS11405 (NWMN_1972)	2190801..2191127	-	327	WP_001052491.1	anti-sigma factor antagonist	-
NWMN_RS11410 (NWMN_1973)	2191247..2192248	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
NWMN_RS11420 (NWMN_1974)	2192598..2192960	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NWMN_RS11425	2192957..2193127	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
NWMN_RS11430 (NWMN_1975)	2193212..2194360	-	1149	WP_001281145.1	alanine racemase	-
NWMN_RS11435 (NWMN_1976)	2194426..2194785	-	360	WP_000581200.1	holo-ACP synthase	-
NWMN_RS11440 (NWMN_1977)	2194789..2195280	-	492	WP_001205910.1	PH domain-containing protein	-
NWMN_RS11445 (NWMN_1978)	2195267..2196850	-	1584	WP_001294631.1	PH domain-containing protein	-
NWMN_RS11450 (NWMN_1979)	2196843..2197322	-	480	WP_001287088.1	hypothetical protein	-
NWMN_RS11455 (NWMN_1980)	2197531..2198091	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T22271 WP_000621175.1 NC_009641:c2192960-2192598 [Staphylococcus aureus subsp. aureus str. Newman]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T22271 NC_009641:c2192960-2192598 [Staphylococcus aureus subsp. aureus str. Newman]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT22271 WP_000948331.1 NC_009641:c2193127-2192957 [Staphylococcus aureus subsp. aureus str. Newman]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT22271 NC_009641:c2193127-2192957 [Staphylococcus aureus subsp. aureus str. Newman]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1