Detailed information of TA system TA222689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2752873..2753618
• Replicon: chromosome
• Accession: NZ_CP086191
• Organism: Xanthomonas oryzae strain JS49-6

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LL928_RS13100
• Protein ID: WP_199285672.1
• Coordinates: 2753289..2753618 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LL928_RS13095
• Protein ID: WP_011407754.1
• Coordinates: 2752873..2753292 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL928_RS13075 (LL928_13065)	2749161..2749487	-	327	Protein_2491	hypothetical protein	-
LL928_RS13080 (LL928_13070)	2749789..2751024	+	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
LL928_RS13085 (LL928_13075)	2751062..2751742	-	681	WP_011257926.1	phosphate phosphatase	-
LL928_RS13090 (LL928_13080)	2751853..2752821	-	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
LL928_RS13095 (LL928_13085)	2752873..2753292	-	420	WP_011407754.1	macro domain-containing protein	Antitoxin
LL928_RS13100 (LL928_13090)	2753289..2753618	-	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
LL928_RS13105 (LL928_13095)	2753721..2755038	+	1318	Protein_2497	IS701-like element ISXo15 family transposase	-
LL928_RS13110 (LL928_13100)	2755082..2755417	-	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LL928_RS13115 (LL928_13105)	2755749..2756675	-	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
LL928_RS13120 (LL928_13110)	2756721..2757512	+	792	WP_011407758.1	polysaccharide deacetylase family protein	-
LL928_RS13130 (LL928_13120)	2757814..2758485	-	672	WP_011407759.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2749789..2755038	5249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T222689 WP_199285672.1 NZ_CP086191:c2753618-2753289 [Xanthomonas oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T222689 NZ_CP118014:c4930843-4930502 [Escherichia coli]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT222689 WP_011407754.1 NZ_CP086191:c2753292-2752873 [Xanthomonas oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT222689 NZ_CP118014:c4931209-4930874 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGGCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA