Detailed information of TA system TA222685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 7197..7690
• Replicon: plasmid unnamed1
• Accession: NZ_CP086189
• Organism: Xanthomonas oryzae strain PXO79

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LL930_RS23495
• Protein ID: WP_274393981.1
• Coordinates: 7197..7523 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LL930_RS23500
• Protein ID: WP_017157755.1
• Coordinates: 7520..7690 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL930_RS23485 (LL930_23460)	2600..4174	+	1575	WP_005917865.1	type IV secretion system DNA-binding domain-containing protein	-
LL930_RS23490 (LL930_23465)	4188..7127	+	2940	WP_274393980.1	MobF family relaxase	-
LL930_RS23495 (LL930_23470)	7197..7523	-	327	WP_274393981.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LL930_RS23500 (LL930_23475)	7520..7690	-	171	WP_017157755.1	antitoxin MazE family protein	Antitoxin
LL930_RS23505 (LL930_23480)	8027..8446	-	420	WP_011052981.1	hypothetical protein	-
LL930_RS23510 (LL930_23485)	8572..9450	-	879	WP_274393988.1	transglycosylase SLT domain-containing protein	-
LL930_RS23515 (LL930_23490)	9461..10483	-	1023	WP_082340387.1	P-type DNA transfer ATPase VirB11	-
LL930_RS23520 (LL930_23495)	10494..11714	-	1221	WP_274393982.1	TrbI/VirB10 family protein	-
LL930_RS23525 (LL930_23500)	11716..12498	-	783	WP_274393983.1	P-type conjugative transfer protein VirB9	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38590	38590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11660.75 Da
• Isoelectric Point: 10.1008

>T222685 WP_274393981.1 NZ_CP086189:c7523-7197 [Xanthomonas oryzae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRIDADALVVIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T222685 NZ_CP118014:4245774-4245992 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6518.34 Da
• Isoelectric Point: 4.1476

>AT222685 WP_017157755.1 NZ_CP086189:c7690-7520 [Xanthomonas oryzae]
MAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT222685 NZ_CP118014:4245374-4245748 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG