Detailed information of TA system TA222671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4124065..4124810
• Replicon: chromosome
• Accession: NZ_CP086183
• Organism: Xanthomonas oryzae strain PXO341

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LL922_RS19260
• Protein ID: WP_199285672.1
• Coordinates: 4124065..4124394 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LL922_RS19265
• Protein ID: WP_011407754.1
• Coordinates: 4124391..4124810 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL922_RS19230 (LL922_19195)	4119196..4119867	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
LL922_RS19240 (LL922_19205)	4120169..4120960	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
LL922_RS19245 (LL922_19210)	4121006..4121932	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
LL922_RS19250 (LL922_19215)	4122264..4122599	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LL922_RS19255 (LL922_19220)	4122643..4123962	-	1320	WP_011407756.1	IS701 family transposase	-
LL922_RS19260 (LL922_19225)	4124065..4124394	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
LL922_RS19265 (LL922_19230)	4124391..4124810	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
LL922_RS19270 (LL922_19235)	4124862..4125830	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
LL922_RS19275 (LL922_19240)	4125965..4126621	+	657	WP_075246166.1	phosphate phosphatase	-
LL922_RS19280 (LL922_19245)	4126659..4127894	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
LL922_RS19285 (LL922_19250)	4128196..4128522	+	327	Protein_3700	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4122643..4127894	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T222671 WP_199285672.1 NZ_CP086183:4124065-4124394 [Xanthomonas oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T222671 NZ_CP118014:897825-898352 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT222671 WP_011407754.1 NZ_CP086183:4124391-4124810 [Xanthomonas oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT222671 NZ_CP118014:897552-897818 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA