Detailed information of TA system TA222644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4034003..4034631
• Replicon: chromosome
• Accession: NZ_CP086177
• Organism: Streptomyces fungicidicus strain F7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LL040_RS18275
• Protein ID: WP_121546814.1
• Coordinates: 4034221..4034631 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D9XL36
• Locus tag: LL040_RS18270
• Protein ID: WP_004928730.1
• Coordinates: 4034003..4034224 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL040_RS18245	4030103..4030369	+	267	WP_121546811.1	CopG family transcriptional regulator	-
LL040_RS18250	4030366..4030809	+	444	WP_121546812.1	PIN domain-containing protein	-
LL040_RS18255	4031418..4032245	+	828	WP_228313141.1	DUF2182 domain-containing protein	-
LL040_RS18260	4032332..4032988	-	657	WP_121546813.1	DUF1326 domain-containing protein	-
LL040_RS18265	4033502..4033657	+	156	WP_228313001.1	hypothetical protein	-
LL040_RS18270	4034003..4034224	+	222	WP_004928730.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LL040_RS18275	4034221..4034631	+	411	WP_121546814.1	PIN domain-containing protein	Toxin
LL040_RS18280	4034665..4034941	+	277	Protein_3594	integrase	-
LL040_RS18285	4035714..4036077	+	364	Protein_3595	pyridine nucleotide-disulfide oxidoreductase	-
LL040_RS18290	4036388..4036921	+	534	WP_121546815.1	DUF5994 family protein	-
LL040_RS18295	4036889..4037284	-	396	WP_203550461.1	DUF5994 family protein	-
LL040_RS18300	4037600..4038190	+	591	WP_033276641.1	DUF5994 family protein	-
LL040_RS18305	4038196..4038477	+	282	WP_033276642.1	hypothetical protein	-
LL040_RS18310	4038474..4039514	-	1041	WP_121546816.1	acyl-CoA desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-121)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-42)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15031.82 Da
• Isoelectric Point: 6.2112

>T222644 WP_121546814.1 NZ_CP086177:4034221-4034631 [Streptomyces fungicidicus]
VIYLLDTSGLVRLLRDPKLQSAWYDAIDAGAIASCYVQRAEFLHSARNGREYDEITEMFTDLYPDVSVPKNAGRWIGAVQ
HRMARAGEHRSASAVDLLIAATAAHHGLAVLHDDADYRAIARHASDLTEHNVHDIA

Nucleotide

• Length: 411 bp

>T222644 NZ_CP117979:c2432885-2432523 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8561.59 Da
• Isoelectric Point: 7.3209

>AT222644 WP_004928730.1 NZ_CP086177:4034003-4034224 [Streptomyces fungicidicus]
MSVTQIDIDDDALERAMALAHVRTKKEAVNLALNFYAEQQERAARISRHFERAREWGAVEDAERLHRAEKRNR

Nucleotide

• Length: 222 bp

>AT222644 NZ_CP117979:c2433052-2432882 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: D9XL36