Detailed information of TA system TA222622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 37435..38096
• Replicon: plasmid pBb144S4c
• Accession: NZ_CP086139
• Organism: Bradyrhizobium barranii subsp. barranii strain 144S4

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J4G43_RS54645
• Protein ID: WP_208089683.1
• Coordinates: 37689..38096 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J4G43_RS54640
• Protein ID: WP_208089682.1
• Coordinates: 37435..37689 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G43_RS54615 (J4G43_054795)	32525..34612	+	2088	WP_049810452.1	recombinase family protein	-
J4G43_RS54620 (J4G43_054800)	34600..34908	-	309	WP_060907863.1	IS66 family insertion sequence element accessory protein TnpB	-
J4G43_RS54625 (J4G43_054805)	34905..35303	-	399	WP_026192391.1	transposase	-
J4G43_RS54630 (J4G43_054810)	35481..36167	-	687	WP_038946061.1	SMC-Scp complex subunit ScpB	-
J4G43_RS54635 (J4G43_054815)	36185..37105	-	921	WP_225006054.1	DUF1403 family protein	-
J4G43_RS54640 (J4G43_054820)	37435..37689	+	255	WP_208089682.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J4G43_RS54645 (J4G43_054825)	37689..38096	+	408	WP_208089683.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4G43_RS54650 (J4G43_054830)	38093..39190	+	1098	WP_208089684.1	tyrosine-type recombinase/integrase	-
J4G43_RS54655 (J4G43_054835)	39332..39598	+	267	WP_035679268.1	type II toxin-antitoxin system VapB family antitoxin	-
J4G43_RS54660 (J4G43_054840)	39598..40020	+	423	WP_035679271.1	type II toxin-antitoxin system VapC family toxin	-
J4G43_RS54665 (J4G43_054845)	40230..41549	-	1320	WP_038381010.1	plasmid replication protein RepC	-
J4G43_RS54670 (J4G43_054850)	41719..42738	-	1020	WP_208089685.1	plasmid partitioning protein RepB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..140264	140264
	-	flank	IS/Tn	-	-	34600..34908	308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-68)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14754.83 Da
• Isoelectric Point: 6.0778

>T222622 WP_208089683.1 NZ_CP086139:37689-38096 [Bradyrhizobium barranii subsp. barranii]
MYLIDTNVVSEARRGSPQATGWLRSVDPASVYLSTLTLGEIMRGIALKQKSDPKVAGHLAEWLRKLRHDHANRILAVTDQ
ISVEWGRIAAIRPCGDIDGLIAATAIVHDLILVTRNVGDFEDTGATVINPWEDSA

Nucleotide

• Length: 408 bp

>T222622 NZ_CP117974:5393687-5393968 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9323.63 Da
• Isoelectric Point: 5.0790

>AT222622 WP_208089682.1 NZ_CP086139:37435-37689 [Bradyrhizobium barranii subsp. barranii]
MEWPLQDAKNQFSKVVQKARLEGPQVVTLRGMRAAVVLSAADYDALRSGRPTLVDDLLAGPSWDDELAEEAMRRIKPPSR
DAAF

Nucleotide

• Length: 255 bp

>AT222622 NZ_CP117974:5393463-5393690 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA