Detailed information of TA system TA222620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 86028..86668
• Replicon: plasmid pBb144S4b
• Accession: NZ_CP086138
• Organism: Bradyrhizobium barranii subsp. barranii strain 144S4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A939MFM8
• Locus tag: J4G43_RS53660
• Protein ID: WP_028153008.1
• Coordinates: 86028..86414 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Z0QMX7
• Locus tag: J4G43_RS53665
• Protein ID: WP_028153009.1
• Coordinates: 86414..86668 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G43_RS53640 (J4G43_053805)	82246..82644	+	399	WP_026192391.1	transposase	-
J4G43_RS53645 (J4G43_053810)	82641..83012	+	372	WP_208086045.1	IS66 family insertion sequence element accessory protein TnpB	-
J4G43_RS53650 (J4G43_053815)	83106..84650	+	1545	WP_224517700.1	IS66 family transposase	-
J4G43_RS53655 (J4G43_053820)	84952..85973	-	1022	Protein_90	tyrosine-type recombinase/integrase	-
J4G43_RS53660 (J4G43_053825)	86028..86414	-	387	WP_028153008.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4G43_RS53665 (J4G43_053830)	86414..86668	-	255	WP_028153009.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J4G43_RS53670 (J4G43_053835)	86779..87186	-	408	WP_028153010.1	type II toxin-antitoxin system VapC family toxin	-
J4G43_RS53675 (J4G43_053840)	87186..87440	-	255	WP_028153011.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
J4G43_RS53680 (J4G43_053845)	87748..88686	+	939	Protein_95	DUF1403 family protein	-
J4G43_RS53685 (J4G43_053850)	88688..89164	+	477	Protein_96	SMC-Scp complex subunit ScpB	-
J4G43_RS53690 (J4G43_053855)	89290..90632	+	1343	Protein_97	IS1380-like element ISBdi2 family transposase	-
J4G43_RS53695 (J4G43_053860)	90802..91040	+	239	Protein_98	hypothetical protein	-
J4G43_RS53700 (J4G43_053865)	91319..91666	+	348	WP_028181697.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..248585	248585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13718.57 Da
• Isoelectric Point: 5.5902

>T222620 WP_028153008.1 NZ_CP086138:c86414-86028 [Bradyrhizobium barranii subsp. barranii]
MVIDSSAILAIFLNEPEASEFEHRIAADPVRLISAATVLETGIGIESRLGDGGGGELDLWLHKVGVEIVPVTAGHADQAR
RAWRRYGKGRHPAKLNFGDCFSYALAALTGEPLLFKGSDFSQTDIKAA

Nucleotide

• Length: 387 bp

>T222620 NZ_CP117974:c2747787-2747536 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9317.71 Da
• Isoelectric Point: 4.8660

>AT222620 WP_028153009.1 NZ_CP086138:c86668-86414 [Bradyrhizobium barranii subsp. barranii]
MALSIKDPETEKLARTLAERTGETITVATRRAIEDRLRRIGADATKAALIEDMEAIRRRLAALPVLDPRSMDDILGYDEN
GLPV

Nucleotide

• Length: 255 bp

>AT222620 NZ_CP117974:c2747539-2747285 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z0QMX7