Detailed information of TA system TA222619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 92069..92609
• Replicon: plasmid pBb144S4a
• Accession: NZ_CP086137
• Organism: Bradyrhizobium barranii subsp. barranii strain 144S4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J4G43_RS52180
• Protein ID: WP_208089796.1
• Coordinates: 92069..92407 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J4G43_RS52185
• Protein ID: WP_208089797.1
• Coordinates: 92394..92609 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G43_RS52165 (J4G43_052315)	87674..87868	+	195	WP_157790354.1	hypothetical protein	-
J4G43_RS52170 (J4G43_052320)	87865..90327	+	2463	WP_208089794.1	AAA family ATPase	-
J4G43_RS52175 (J4G43_052325)	90412..91530	+	1119	WP_208089795.1	restriction endonuclease	-
J4G43_RS52180 (J4G43_052330)	92069..92407	-	339	WP_208089796.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J4G43_RS52185 (J4G43_052335)	92394..92609	-	216	WP_208089797.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
J4G43_RS52190 (J4G43_052340)	92964..93194	-	231	WP_208089798.1	hypothetical protein	-
J4G43_RS52195 (J4G43_052345)	93233..93442	-	210	WP_225005936.1	hypothetical protein	-
J4G43_RS52200 (J4G43_052350)	94058..94387	-	330	WP_208089799.1	helix-turn-helix transcriptional regulator	-
J4G43_RS52205 (J4G43_052355)	94780..95364	-	585	WP_208089800.1	hypothetical protein	-
J4G43_RS52210 (J4G43_052360)	95396..95536	+	141	WP_208089801.1	hypothetical protein	-
J4G43_RS52215 (J4G43_052365)	96137..96798	+	662	Protein_93	DNA polymerase IV	-
J4G43_RS52220 (J4G43_052370)	96846..97247	-	402	WP_208089802.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..317868	317868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• PrlF antitoxin (4-54)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12370.37 Da
• Isoelectric Point: 9.8490

>T222619 WP_208089796.1 NZ_CP086137:c92407-92069 [Bradyrhizobium barranii subsp. barranii]
MPAFKQGDVVRVPFPYTDRSTRQHRPALVVSTGGIGENGNLLWVVMITSAENRTWPGDIAISRYEEAGLPAPSIIRPCKI
ATIEARDAERLGRIKPRLMIEVLSEMRGILGS

Nucleotide

• Length: 339 bp

>T222619 NZ_CP117974:c2747539-2747285 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7798.84 Da
• Isoelectric Point: 4.6684

>AT222619 WP_208089797.1 NZ_CP086137:c92609-92394 [Bradyrhizobium barranii subsp. barranii]
MITSKLTTKAQTTIPQAVRTALHLKEGDEIAYTITGDKVVLTKAEPALVDDPFVTFSEWDSEADRKAYASL

Nucleotide

• Length: 216 bp

>AT222619 NZ_CP117974:c2747787-2747536 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA